淫羊藿甙对小鼠T淋巴细胞体外中期和晚期活化的影响  

Effect of icariin on intermediate and advanced activation of murine T lymphocytes in vitro

作　　者：滕菲[1] 曾耀英[1] 黄秀艳[1] 杨志[1] 姚满林[1] 宋兵[1] 李林[1] 

机构地区：[1]暨南大学组织移植与免疫中心,广东广州510632

出　　处：《细胞与分子免疫学杂志》2008年第11期1059-1061,1065,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家重点基础研究发展计划(973)资助项目(2006CB504201;2004CB720100);广东省基金项目(2006B36030016);广州市科技局科技攻关重点项目(2006Z-E0091);生物化学与分子生物学广东省重点学科资助(2008年度)

摘　　要：目的:研究淫羊藿甙(ICA)对刀豆蛋白A(ConA)刺激的小鼠T淋巴细胞体外中期和晚期活化的影响。方法:采用流式细胞术(FCM)结合双色荧光抗体染色技术分别检测中期和晚期活化标志CD25和CD71分子的表达;取中期活化的细胞上清,利用LuminexTM100分析上清中IL-2、IL-4、IL-10、TNF-α、IFN-γ的含量变化。结果:ICA在终浓度0.3、1.5、3.0μmol/L时明显抑制CD25的表达而对CD71没有明显影响;下调Th2型细胞因子及上调Th1型细胞因子的分泌量,但没有剂量依赖效应。结论:ICA下调CD25、IL-2和Th2型细胞因子水平,抑制T细胞活化,同时有一定程度的促进细胞免疫反应,因此ICA可能具有双向调节免疫平衡的作用。AIM： To study the effect of icariin （ICA） on the intermediate and advanced activation of murine T lymphocytes stimulated by concanvalin A （ConA） in vitro. METHODS： Single cell suspensions were prepared from murine lymphoid nodes under germ free condition. The drug toxicity of ICA on T lymphocytes was measured by MTT after 24 h. Before they were stimulated by ConA （the final concentration was 5 mg/L）, T cells were treated with different concentrations of icariin for 4 h. The expression of CD25 and CD71, the marker of intermediate and advanced activation of T lymphocytes, were measured by flow cytometry （FCM） combined with two-colour immunofluorescent staining of cell surface antigen after 24 h and 30 h. The change of cytokine contents such as IL-2, IL-4, IL-10, TNF-α and IFN-γ in the supernatant of intermediate activation was measured by LuminexTM100. RESULTS： ICA at the final concentration of 0.3, 1.5, 3.0 μmol/L inhibited the expression of CD25 with dose-dependent effect but it had no effect on that of CD71. ICA decreased the secretion of IL-2, IL-4 and IL-10 but increased that of TNF-α and IFN-γ. CONCLUSION： ICA can not only decrease CD25, IL-2, IL-4 and IL-10 but also enhance the cell-mediated immunity reaction. So it may have a function of two-way regulation of immune balance.

关 键 词：淫羊藿甙 T淋巴细胞 CD25 CD71 流式细胞术 

分 类 号：R363[医药卫生—病理学]