偏侧帕金森病大鼠模型的行为、免疫组化和脑微透析的实验研究  

Study on 6-OHDA-Induced Rat Model of Hemiparkinsonism:Behavior,Imniunohistochemistry and Microdialysis

作　　者：赵迎春[1] 刘振国[1] 陈生弟[1] 余慧贞[1] 蒋芝华[2] 周长福[2] 

机构地区：[1]上海第二医科大学瑞金医院神经内科 [2]中国科学院上海生理研究所,200031

出　　处：《临床神经科学》1997年第3期148-152,共5页Chinese Journal of Clinical Neurosciences

基　　金：国家自然科学基金(39470261); 上海市科委青年科技启明星后计划(94QMH1402)

摘　　要：评价6-羟多巴胺(6-OHDA)损毁大鼠单侧黑质制备的偏侧帕金森病动物模型。应用6-羟多巴胺损毁SD大鼠单侧黑质制备偏侧PD鼠模型。3周后根据药物诱发试验,TH免疫组化证实模型制作成功。进一步用脑微透析技术结合HPLC-ECD在体检测PD鼠纹状体多巴胺及代谢产物含量。结果:82只大鼠中有36只阿朴吗啡(APO)诱发的旋转次数>7转/min。6-OHDA注射侧黑质DA神经末稍已绝大多数被损毁。6-OHDA损毁侧纹状体多巴胺及代谢产物明显低于健侧(P<0.05,P<0.01)。应用6-OHDA制备的偏侧PD鼠模型是PD研究的理想模型之一。To evaluate the hemiparkinsonian rats induced by 6-OHDA,a rat model of hemiparkin-sonism was induced by unilateral administration of 6-hydroxydopamine (6-OHDA) into the substantia nigra. 6-OHDA-treated rats were tested for apomorphin(APO)-induced rotational asymmetry and TH immunohistochemistry analysis. Extracellular DA and its metabolites (DOPAC.HVA) in the striaturn of hemiparkinsonian rat model were measured by microdia-lysis and high performance liquid chromatography with electrochemical detection (HPLC-ECD). 36 of the total 82 rats exhibiting the contralateral rotation(>7 turns/min) were selected for further study. Dopaminergic neurons were almost absent in the lesioned nigra and TH-positive fibers were not observed in the denervated striatum. The levels of DA, DOPAC and HVA in the lesioned side were significantly decreased (P<0. 05, P<0. 01 and P<0. 01),as compared with those in the intact side. The hemiparkinsonian rat induced by 6-OH- DA provides a very useful model for the study of human Parkinson's disease.

关 键 词：震颤麻痹 动物模型 旋转行为 免疫组化 微透析 

分 类 号：R742.502[医药卫生—神经病学与精神病学]