RhoC过表达对人脐静脉内皮细胞体外迁移及血管生成能力的影响  

Overexpression of RhoC Affects Human Umbilical Vein Endothelial Cell Migration and Angiogenesis in Vitro

作　　者：赵良平[1] 张庆华[1] 王薇娜[2] 田训[1] 梁逢奇[1] 黄磊[1] 熊国平[1] 马丁[3] 

机构地区：[1]武汉市中心医院妇产科,430014 [2]武汉大学中南医院心内科 [3]华中科技大学同济医学院附属同济医院妇产科

出　　处：《肿瘤防治研究》2008年第11期766-769,共4页Cancer Research on Prevention and Treatment

基　　金：国家重点基础研究发展规划973项目(2002CB513100)

摘　　要：目的探讨RhoC基因过量表达对内皮细胞体外迁移及血管生成过程的影响。方法构建RhoC真核表达载体,以脂质体转染人脐静脉内皮细胞(HUVE),采用RT-PCR和Western blot检测RhoC的mRNA及蛋白表达水平;划痕实验检测细胞迁移能力;胶原基质胶三维培养检测内皮细胞体外血管生成能力。结果转染RhoC实验组与空载体对照组比较,RhoC mRNA和蛋白表达明显增高;细胞迁移能力和血管样结构生成能力明显增强。结论RhoC高表达能促进人脐静脉内皮细胞体外迁移及血管生成。Objective To investigate the effects of RhoC overexpression on human umbilical vein endothelial（HUVE） cell migration and angiogenesis in vitro. Methods Transfecting RhoC gene into HUVE cells by Lipofectamine-2000. The expression of RhoC mRNA and protein were detected respectively by RT-PCR and Western blot, respectively. Wound assay in vitro and three-dimensional culture were used to detect the migration and angiogenesis capacity of HUVE. Results RhoC expression was increased by trans-fecting RhoC gene into HUVE, meanwhile, the cells migration and angiogenesis capacity were enhanced. Conclusion RhoC may increase the capacity of HUVE migration and angiogenesis in vitro.

关 键 词：RHOC 人脐静脉内皮细胞 细胞迁移 血管形成 

分 类 号：Q786[生物学—分子生物学] R73-37[医药卫生—肿瘤]