不同硒源和水平对蛋鸡脾硒含量及免疫功能的影响  

Effect of Selenium Source and Level in Diets on Spleen Selenium Content and Immune Function of Laying Hen

作　　者：潘翠玲[1] 黄克和[1] 赵玉鑫[1] 陈甫[1] 焦淼[1] 饶静静[1] 

机构地区：[1]南京农业大学动物医学院畜禽营养代谢病研究室,南京210095

出　　处：《畜牧兽医学报》2008年第11期1523-1529,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目资助(30371060;30671547);博士点科学基金项目资助(20070307005)

摘　　要：将富硒益生菌(Se-enriched probiotics,SP)和亚硒酸钠(Sodium selenite,SS)2种硒源分别以3个硒水平0.2、0.5和1.0 mg/kg添加到蛋鸡基础日粮,进行为期35 d的饲养试验。结果表明,日粮添加SP或SS均能显著提高脾硒含量,随着硒添加水平的升高,脾硒含量也显著升高,且添加SP较SS能显著提高脾硒含量;在试验的第14天,添加SS或SP均能显著促进蛋鸡外周血T淋巴细胞转化,且随着硒添加水平的升高,T淋巴细胞的转化增加;在试验的第14和第28天,添加SS或SP均能显著提高蛋鸡血浆IL-2的水平,随着硒添加水平的升高和试验时间的延长,血浆IL-2均升高,且在试验的第28天,添加SP较添加SS能显著提高蛋鸡血浆IL-2的水平。结论:日粮添加SS或SP均能提高蛋鸡的免疫能力,且随硒添加水平的升高效果愈好;随着试验时间的延长,添加SP的效果优于SS。Se sources （Se-enriched probiotics, SP and sodium selenite, SS）were added into basal diet at 0. 2, 0.5 and 1.0 mg/kg of Se. Feeding test lasted for 35 days. The results showed that the addition of either SS or SP significantly increased Se content in spleen. With an increase of the supplemental Se level, the Se content in spleen had a significant increase. Supplemental SP had a more significant action to increase Se content in spleen than SS. On days 14, supplemental Se from either SS or SP significantly promoted the T lymphocyte transformation in hen peripheral blood. With supplemental Se level increase, the T lymphocyte transformation were increased. On days 14 and 28, supplemental Se from either SS or SP significantly increased the plasma IL-2 level of hen. With supplemental Se level increase and the extension of experiment duration, the plasma IL-2 level also significantly increased. On days 28, there was a more significant increase in the plasma IL-2 level of hens fed by SP than that of hens fed by SS. The conclusion is that supplemental Se from either SS or SP significantly increased Se content in spleen, significantly promoted immune function of hen. With supplemental Se level increase, the effect was better. With the extension of experiment duration, supplemental SP had a better effect than SS.

关 键 词：富硒益生菌 亚硒酸钠 蛋鸡 脾脏 硒含量 淋巴细胞转化 IL-2 

分 类 号：S816.7[农业科学—饲料科学] S858.310[农业科学—畜牧学]