Reduced cell death by inosine pretreatment after photochemically induced cerebral ischemia in adult rats  

作　　者：Zhongliang Wu Fei Liu Yazhou Wang Xiying Jiao Ming Shi Qingbo Zhao Gong Ju Siwei You 

机构地区：[1]Institute of Neurosciences, The Fourth Military Medical University, 17 Changlexi Road, Xi＇an 710032, China [2]Department of Biostatistics, The Fourth Military Medical University, 17 Changlexi Road, Xi＇an 710032, China

出　　处：《Progress in Natural Science:Materials International》2008年第12期1513-1518,共6页自然科学进展·国际材料（英文版）

基　　金：supported by the research grants from National Natural Science Foundation of China (30571998);the National Program on Key Basic Research Projects (2003CB515031)

摘　　要：Protective effect of inosine against cell death after photochemically induced focal cerebral ischemia was investigated in adult rats. Intraperitoneal injection of inosine was started 24 h before operation and continued till the animals were sacrificed. The TUNEL method and Nissl staining were used to identify apoptosis and necrosis, respectively. TUNEL-positive cells were located in the transitional zone between the necrotic core and normal cortex. The mean maximum area of the necrotic cores and mean number of TUNEL-positive cells 24 h after operation were reduced significantly in the inosine-treated rats when compared with those in the saline-treated control animals. We suggest that inosine can significantly reduce both necrosis and apoptosis following cerebral ischemia induced by photothrombosis in adult rats.对在相片以后的房间死亡的肌甙的保护的效果化学上导致了焦点的服的局部缺血在成年老鼠被调查。肌甙的 Intraperitoneal 注射被开始在操作前的 24 h 并且继续直到动物被牺牲。TUNEL 方法并且 Nissl 染色被用来分别地识别 apoptosis 和坏死。TUNEL 积极的房间位于在坏死的核心和正常外皮之间的过渡地区。坏死的核心的吝啬的最大的区域和 TUNEL 积极的房间的吝啬的数字当时，在操作以后的 24 h 在对待肌甙的老鼠显著地被减少与那些相比在盐对待的控制动物。我们建议那肌甙能显著地减少服的局部缺血在成年老鼠由相片血栓导致了的坏死和 apoptosis 追随者。

关 键 词：Cell death Cerebral infarction INOSINE Photothrombosis  Rat 

分 类 号：R742[医药卫生—神经病学与精神病学]