新疆准噶尔铁线莲不同部位总黄酮提取工艺的研究  

Study on Extraction Technology of Total Flavonoids from Different Parts of Clematis songarica Bunge in Xinjiang

作　　者：古丽巴哈尔.阿巴拜克力 

机构地区：[1]新疆师范大学生命科学与化学学院生物系,新疆乌鲁木齐830054

出　　处：《食品科学》2008年第11期365-368,共4页Food Science

基　　金：新疆师范大学优秀青年教师科研启动基金项目(XJNU0726)

摘　　要：采用分光光度法,以芦丁为标准样品测定新疆准噶尔铁线莲不同部位中的总黄酮的含量,采用正交试验设计对其不同部位中总黄酮提取工艺进行最佳筛选,四种因素对不同部位总黄酮含量测定均有影响,分别得到的最佳提取工艺条件为:茎:乙醇浓度50%,温度50℃,浸提时间3h,料液比1:50,吸光度0.270;叶:乙醇浓度60%,温度70℃,浸提时间3h,料液比1:30,吸光度0.562。花:乙醇浓度50%,温度60℃,时间2h,料液比1:50,吸光度0.480;果实:乙醇浓度60%,温度70℃,浸提时间3h,料液比1:40,吸光度0.365。In this study, the total flavonoids content was assayed by using spectrophotometry method with rutin as standard. The extraction technology of the total flavonoids from different parts of Clematis songarica Btmge, such as stem, leaf, flower and fruit was optimized by means of orthogonal test. Results showed that for the stem, the optimal extraction conditions are： ethanol concentration 50%, extraction temperature 50 ℃, extraction time 3 h and ratio of material to ethanol solution 1：50, and the absorbance of the total flavonoids extract is 0.270 under the optimal conditions. For the leaf, the optimal extraction condition are： ethanol concentration 60%, extraction temperature 70 ℃, extraction time 3 h and ratio of material to ethanol solution 1：30, and the absorbance of the total flavonoids extract is 0.562 under the optimal conditions. For the flower, the optimal extraction conditions are： ethanol concentration 50%, extraction temperature 60 ℃, extraction time 2 h and ratio of material to ethanol solution 1：50, and the absorbance of the total flavonoids extract is 0.480 under the optimal conditions. For the fruit, the optimal extraction conditions are： ethanol concentration 60%, extraction temperature 70 ℃, extraction time 3 h and ratio of material to ethanlo solution 1：40, and the absorbance of the total flavonoids extract is 0.365 under the optimal conditions.

关 键 词：分光光度法 准噶尔铁线莲 总黄酮 含量测定 

分 类 号：TS207.3[轻工技术与工程—食品科学]