液液微萃取技术在糖与糖醇分析检测中的应用  

Application of Liquid-liquid Microextraction in Sugar and Sugar Alcohol Analysis with GC-MS

作　　者：胡磊[1,2] 杨冀艳[1,2] 盖颖[3] 王乐[3] 马莹莹[3] 任静文[3] 蒋湘宁[3] 

机构地区：[1]南昌大学中德联合研究院,江西南昌330047 [2]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [3]北京林业大学生物科学与技术学院,北京100083

出　　处：《食品科学》2008年第11期482-486,共5页Food Science

基　　金：江西省自然科学基金项目(2007GQN2012)

摘　　要：在糖与糖醇传统前处理方法的基础上,分别研究了糖与糖醇的半微量和微量前处理方法,采用气相色谱质谱联用(GC-MS)技术对衍生化产物进行分析。结果表明,三种前处理方法分析结果相近,但与传统方法相比,后两种方法所用的提取溶剂和衍生化试剂用量大大降低,消耗的时间和人力也大大减少。其中微量前处理方法所用溶剂仅为传统方法的1/200～1/100,处理时间缩短了5倍以上。研究结果表明,毛细管液液微萃取技术(LLME)技术是可行、可靠的,并且具有经济、快速、使用方便等特点。Based on the traditional pretreatment technique, semi-micro-pretreatrnent and micro-pretreatment methods of sugar and sugar alcohol were set up respectively. Gas chromatography and mass spectrometry （GC-MS） were used to analyze the derived products. The results showed that the derived sugars and sugar alcohols from the same sample pretreated with the above-mentioned three methods are nearly the same in gradients and contents. However, compared with the above-mentioned traditional method, the amounts of extraction solvent and derivation solvent used in the other two methods greatly decrease and the time and labour consumed by the other two methods also obviously reduce. The amount of solvent consumed by micro-pretreatment technique is 1/200 to 1/100 of that by traditional pretreatment and the time for pretreatment is decreased above 5 times. Compared with the traditional method, liquid-liquid microextraction （LLME） is economical, fast and convenient.

关 键 词：液液微萃取(LLME) 微量衍生化 糖、 糖醇 气相色谱质谱联用(GC-MS) 毛细管 

分 类 号：TS231[轻工技术与工程—粮食、油脂及植物蛋白工程]