黄孢原毛平革菌固态发酵产漆酶的研究及应用  

Research and Application of Producing Laccase by Phanerochaete chrysosporium in Solid-State Fermentation System

作　　者：彭丹[1] 谢更新[1] 曾光明[1] 陈耀宁[1] 陈芙蓉[1] 胡霜[1] 余震[1] 

机构地区：[1]湖南大学环境科学与工程学院,长沙410082

出　　处：《环境科学》2008年第12期3568-3573,共6页Environmental Science

基　　金：国家重点基础研究发展规划(973)项目(2005CB72403);长江学者和创新团队发展计划项目(IRT0719)

摘　　要：对黄孢原毛平革菌(BKMF-1767)利用香蕉皮和玉米棒为发酵底物产胞外漆酶进行了研究.结果表明,当香蕉皮与玉米棒混合比例为1∶2、诱导剂CuSO4为0.4 mmol/L时,能获得最高漆酶酶活12.68 U/g.利用固态发酵所获得的粗漆酶液,进行了降解五氯酚的试验.在没有氧化还原介体时粗漆酶液能降解PCP,粗酶液中加入5 mmol/L氧化还原介体(ABTS)能获得更高的降解率,反应6 h分别为37.8%和97%.将粗漆酶液用(NH4)2SO4盐析纯化,用提纯后漆酶降解PCP,6 h后降解率为81.8%.The potential of banana skin and corn cob as a support-substrate for the production of extracellular laccase by the white-rot fungus Phanerochaete chrysosporium （BKMF-1767） was investigated. The results indicate that laccasc showed a maximum activity of 12.68 U/g when the proportion of banana skin and corn cob is 1：2 and the inducer is 0.4 mmol/L CuSO4. In addition, crude laccase enzyme shows degradation activity to pentachlorophenol （PCP） without redox mediator or with the redox mediator （ABTS） at a concentration of 5 mmol/L, and the degradation rates of PCP were 37.8 % and 97 % respectively after 6 h. The crude laccase was purified by treatment of （ NH4 ）2 SO4, and the purified laccase could make the degradation rate of PCP to 81.8 % within 6 h.

关 键 词：黄孢原毛平革菌 固态发酵 漆酶 降解 五氯酚 

分 类 号：X172[环境科学与工程—环境科学]