VEGF反义寡核苷酸抑制胆囊癌生长增殖和凋亡的体外实验研究  

The effect of oligofectamine mediated VEGF ASODN transfection on GBC-SD cell growth,proliferation and apoptosis in vitro

作　　者：李海军[1] 毛拉艾沙.买买提 庞作良[1] 

机构地区：[1]新疆医科大学附属肿瘤医院肝胆胰外科,新疆乌鲁木齐830011

出　　处：《新疆医科大学学报》2008年第10期1369-1372,共4页Journal of Xinjiang Medical University

摘　　要：目的:探讨VEGF反义寡核苷酸转染对人胆囊癌GBC-SD细胞生长、增殖和凋亡的影响。方法:运用Oligofectamine介导VEGF反义寡核苷酸(antisense oligodeoxynucleotides,ASODN)和错义寡核苷酸(Scrambled Oligodeoxynucleotide,SODN)转染人胆囊癌(GBC-SD)细胞。采用MTT法测定转染后各组细胞的生长曲线及抑制率,流式细胞术检测转染后不同时间各组细胞凋亡情况。结果:SODN组、SODN十Oligofectamine组对GBC-SD细胞生长无显著影响(P>0.05),ASODN组及ASODN十Oligofectamine组则能显著抑制GBC-SD细胞生长(P<0.05),且ASODN十Oligofectamine组对GBC-SD细胞生长增殖抑制作用较ASODN组更为强(P<0.05)。流式细胞术检测结果发现ASODN十Oligofectamine组能促进GBC-SD细胞凋亡(P<0.05)。结论:VEGFASODN转染能抑制胆囊癌GBC-SD细胞生长和增殖,Oligofectamine介导能明显增强VEGF ASODN的抑制作用;Oligofectamine介导VEGF反义寡核苷酸转染能促进胆囊癌GBC-SD细胞的凋亡。Objective. To investigate the effect of oligofectamine mediated VEGF ASODN transfeetion on GBC-SD cell growth, proliferation and apoptosis in vitro. Methods： GBC-SD cells were transfected VEGF ASODN and SODN mediated by oligofectamine. The transfected GBC-SD cells of each groups were assessed the change of growth and proliferation activity by MTT, and apoptosis by flow cytometry. Results： The transfected GBC-SD cells growth and proliferation activity tested by MTT in groups of SODN and SODN＋otigofeetamine had not any difference as compared with control group （P〉0.05）, but as for the cells in groups of ASODN and ASODN＋oligofectamine, the growth and proliferation activity were significantly inhibited （P〈0.05）, moreover, the effect of ASODN＋oligofectamine was more significant than that of ASODN （P〈0.05）. The results of flow cytometry revealed ASODN＋ oligofectamine could accelerate transfected GBC-SD cells apoptosis （P〈0. 05）. Conclusion： VEGF ASODN could inhibit GBC-SD cells growth, proliferation and accelerate apoptosis. The oligofectamine could strengthen the effect of ASODN.

关 键 词：胆囊癌细胞株GBC—SD VEGF 反义寡核苷酸 Oligifectamine 凋亡 

分 类 号：R735.8[医药卫生—肿瘤]