信号分子对类球红细菌(Rhodobacter sphaeroides碳源利用的影响  

Impact of signal molecule on carbon source utilization of Rhodobacter sphaeroides

作　　者：叶姜瑜[1,2] 李文娟[1] 张英[2,3] 廖强[4] 

机构地区：[1]重庆大学城市建设与环境工程学院,重庆400045 [2]三峡库区生态环境教育部重点实验室,重庆400045 [3]西南大学生命科学学院,重庆400715 [4]重庆大学动力学院工程热物理研究所,重庆400044

出　　处：《生态环境》2008年第5期1991-1995,共5页Ecology and Environmnet

基　　金：国家自然科学基金重点项目(90510020);重庆市自然科学基金项目(2007BB6160);三峡库区水环境安全与生态建设重庆市市级重点实验室开放项目

摘　　要：类球红细菌拥有cer群体感应系统,它既有细胞间信息传递的功能,也对碳源利用进行调节。为调查自诱导物对碳源利用的影响,在仅少量EDTA作唯一能源物的培养基中加入N-(tetradecanoyl)-DL-homoserine lactone,通过测定活菌数变化观察这种类球红细菌自诱导物的结构类似物对碳源利用的影响。然后利用Biolog-GN鉴定板,在加入外源信号分子和对照的条件下,研究了类球红细菌正常培养与饥饿培养下对95种单一碳源的不同利用情况。结果表明,在存在信号分子的饥饿情况下,细菌能更好的利用EDTA维持活菌数量。当培养到6h的时候,加入信号分子培养的鉴定板中,正常培养情况下细菌对碳源的利用丰富度指数最高;而在饥饿培养情况下细菌能利用更多种类的碳源。Cer quorum-sensing system exists in Rhodobacter sphaeroides, which instructs cell-cell communication, and also regulates the utilization of carbon sources. To investigate the effect of inducer on the utilization of carbon sources, N-（tetradecanoyl）-DL-homoserine lactone, a structure analog of the signal molecule of R. sphaeroides, was added into the medium which contained a small quantity of EDTA as only carbon source. Used Biolog-GN identification plates, in exogenous signal molecule being added in or not, the utilization of 95 single carbon sources were studied under control culture and starvation. The results showed that the bacterium can make a good use of EDTA to maintain a survival under starvation. After 6 hours cultivation, the abundance index of carbon sources utilization was the highest under normal culture; contrast to this, more types of carbon sources were used under starvation culture.

关 键 词：自体诱导物 碳源 饥饿 类球红细菌 BIOLOG 

分 类 号：Q938.1[生物学—微生物学]