慢性EAE模型C57BL/6J小鼠脑和脊髓EBI3和p28 mRNA表达  

Expression of EBI3 and p28 mRNA in the brain and spinal cord of chronic model of experimental autoimmune encephalomyelitis in C57BL/6J mice

作　　者：颜津津[1] 王瑞[1] 王玉忠[1] 周文斌[1] 

机构地区：[1]中南大学湘雅医院神经内科,长沙410008

出　　处：《中南大学学报（医学版）》2008年第11期1028-1036,共9页Journal of Central South University ：Medical Science

基　　金：湖南省科技厅资助项目(2007SK3032)~~

摘　　要：目的:观察IL-27两个亚单位EBI3 mRNA和p28 mRNA在慢性实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)模型C57BL/6J小鼠脑和脊髓动态表达的变化,探讨其在EAE发病机制中的作用。方法:72只近交系无特定病原体的6～8周健康雌性C57BL/6J小鼠随机分为空白对照组、佐剂组和慢性EAE模型组,采用RT-PCR技术观察不同时期脑和脊髓EBI3 mRNA和p28 mRNA的表达变化。结果:EAE组小鼠在发病初期即有EBI3 mRNA和p28 mRNA的表达升高,在疾病高峰期迅速增加,并在慢性期仍维持在较高水平;与空白对照组和佐剂组相比较,差异均有统计学意义(P<0.01)。空白对照组小鼠脑和脊髓EBI3 mRNA和p28 mRNA的表达与佐剂组相比较,差异无统计学意义(P>0.05)。结论:IL-27有可能在早期促进EAE发病,并维持最后阶段的炎症反应。Objective To investigate the expression of EBI3 and p28 mRNA （ the 2 subunits of IL-27 ） in the brain and spinal cord of the model of experimental autoimmune encephalomyelitis （EAE） , and to explore their effect on EAE. Methods Seventy-two adult female SPF C57 BL/6J mice（ inbred strain ） were randomly divided into a control group, an adjuvant group, and an EAE group. RT-PCR was performed to detect the expression of EBI3 mRNA and p28 mRNA in the brain and spinal cord. Results The expression of EBI3 mRNA and p28 mRNA was up-regulated at onset in the EAE group, which increased quickly during peak phase and maintained at a high level in the chronic phase. There was significant difference in the expression of EBI3 and p28 mRNA between the EAE group and the control/adjuvant group （ P 〈 0.01 ）. Additionally, there was no remarkable difference in the expression of EBI3 and p28 mRNA in the brain and spinal cord between the control group and the adjuvant group （ P 〉 0.05 ）. Conclusion IL-27 may play a role of promoting the morbility of EAE in the early stage, and sustain the inflammatory response in endgame.

关 键 词：实验变态反应性脑脊髓炎 IL-27 EBI3 p28 动物模型 

分 类 号：R744[医药卫生—神经病学与精神病学]