Angiopoietin-1 mRNA expression in estradiol-treated ovariectomized rats with focal cerebral ischemia after reperfusion  

作　　者：Ling Tang Jun Zuo Yonghong Wang Yuanda Zhou Haixia He 

机构地区：[1]Department of Neurology, the Second People＇s Hospital of Chongqing, Chongqing 402160, China [2]Department of Pharmacy, First People＇s Hospital of Beijiang District, Chongqing 400020, China [3]Department of Neurology, First Hospital of Chongqing Medical University, Chongqing 400016, China [4]Research Laboratory of Clinical Pharmacology, First Hospital of Chongqing Medical University, Chongqing 400016, China

出　　处：《Neural Regeneration Research》2008年第10期1078-1083,共6页中国神经再生研究（英文版）

基　　金：Supported by: the Natural Science Foundation of Chongqing, No. CSTC2006EB5030

摘　　要：BACKGROUND： Epidemiologic studies have indicated that the incidence of stroke in premenopausal females is lower than in males at the same age, but it significantly rises in postmenopansal females. Estrogen is used clinically to alleviate injury caused by cerebral ischemia, it has been hypothesized that the neuroprotective role of estrogen relates to angiopoietin （Angpt）, which plays an important role in vascularization, vascular remodeling and maturation. OBJECTIVE： To observe and validate the effect of estradiol on angiopoietin-1 （Angptl） mRNA expression in ovariectomized rats with focal cerebral ischemia after reperfusion, so as to explore the molecular mechanisms of estradiol-mediated protection from cerebral ischemic damage. DESIGN, TIME AND SETTING： Randomized, controlled, molecular biology, prospective animal study. The experiment was performed at the Central Laboratory of Chongqing Medical University from September to December 2005. MATERIALS： Fifty healthy female wild type （WT） rats aged 6 months and fifty female rats aged 6 months with knockout of the estrogen-alpha receptor gene （ERKO）. METHODS： WT rats and ERKO rats were divided into estradiol and control groups （n = 25）, and injected intramuscularly with estradiol benzoate （100μg/kg per day） or corn oil （l mL/kg per day） for 7 days, 30 days after bilateral ovariectomy. Rat models of cerebral ischemia/reperfusion were established with the middle cerebral artery occlusion method. After 30 minutes of middle cerebral artery occlusion, rats from the estradiol and control groups were injected intramuscularly with estradiol benzoate or corn oil at the above dose. MAIN OUTCOME MEASURES： We used radio-immunity analysis and laser-Doppler flowmetry to measure plasma estradiol levels and changes in cerebral blood flow. We used immunohistochemical staining of CD34 epitopes to measure changes in the capillary density in brain following cerebral iscbemia/reperfusion, and quantitative RT-PCR analysis to assess mRNA expression BACKGROUND： Epidemiologic studies have indicated that the incidence of stroke in premenopausal females is lower than in males at the same age, but it significantly rises in postmenopansal females. Estrogen is used clinically to alleviate injury caused by cerebral ischemia, it has been hypothesized that the neuroprotective role of estrogen relates to angiopoietin （Angpt）, which plays an important role in vascularization, vascular remodeling and maturation. OBJECTIVE： To observe and validate the effect of estradiol on angiopoietin-1 （Angptl） mRNA expression in ovariectomized rats with focal cerebral ischemia after reperfusion, so as to explore the molecular mechanisms of estradiol-mediated protection from cerebral ischemic damage. DESIGN, TIME AND SETTING： Randomized, controlled, molecular biology, prospective animal study. The experiment was performed at the Central Laboratory of Chongqing Medical University from September to December 2005. MATERIALS： Fifty healthy female wild type （WT） rats aged 6 months and fifty female rats aged 6 months with knockout of the estrogen-alpha receptor gene （ERKO）. METHODS： WT rats and ERKO rats were divided into estradiol and control groups （n = 25）, and injected intramuscularly with estradiol benzoate （100μg/kg per day） or corn oil （l mL/kg per day） for 7 days, 30 days after bilateral ovariectomy. Rat models of cerebral ischemia/reperfusion were established with the middle cerebral artery occlusion method. After 30 minutes of middle cerebral artery occlusion, rats from the estradiol and control groups were injected intramuscularly with estradiol benzoate or corn oil at the above dose. MAIN OUTCOME MEASURES： We used radio-immunity analysis and laser-Doppler flowmetry to measure plasma estradiol levels and changes in cerebral blood flow. We used immunohistochemical staining of CD34 epitopes to measure changes in the capillary density in brain following cerebral iscbemia/reperfusion, and quantitative RT-PCR analysis to assess mRNA expression

关 键 词：cerebral ischemia/reperfusion ESTROGEN ANGIOPOIETIN model animal 

分 类 号：R742[医药卫生—神经病学与精神病学]