真姬菇多糖的分离纯化及结构分析  

Extraction,purification and structural characterization of hypsizigus marmoreus polysaccharides

作　　者：姜华[1] 纪春暖[1] 陈健[1] 蔡德华[2] 

机构地区：[1]鲁东大学化学与材料科学学院,烟台264025 [2]鲁东大学生命科学学院,烟台264025

出　　处：《食品科技》2008年第12期204-207,共4页Food Science and Technology

基　　金：科技部星火计划项目(2006EA740001);烟台市科学技术发展计划项目(2006148);鲁东大学研究生教育项目(YD05002);鲁东大学创新团队建设项目

摘　　要：以真姬菇(Hypsizigus marmoreus)子实体为材料提取粗多糖。用Sevage法脱蛋白,活性炭脱色,经DEAE-纤维素柱(D3.5 cm×60 cm)层析得真姬菇纯化多糖HPS-Ⅰ和HPS-Ⅱ。经SephadexG-200凝胶柱(D1.6×30 cm)层析,证明HPS-Ⅱ为单一组分。元素分析结果表明,HPS-Ⅱ不含氮、磷、硫,碳和氢的含量分别是37.81%和6.737%。采用Sepharose CL-4B凝胶柱(D1.6×30 cm)层析,以Dextran多糖为标准品,测得HPS-Ⅱ的平均分子量为4.61×105。经红外光谱和1H NMR和13C NMR谱分析确定HPS-Ⅱ为以(1→4)-α-D-Glep为主链,(1→6)-α-D-Glep为侧链的α-D-吡喃葡聚糖。The crude polysaccharides were extracted from Hypsizigus marmoreus fruit bodies. The purified polysaccharides HPS- Ⅰ and HPS- Ⅱ were isolated from it by extracting protein with Sevage method, decoloring with active carbon, DEAE cellulose column chromatography（D3.5 cm×60 cm）. HPS- Ⅱ was proved to be homogeneous by Sephadex G-200 column chromatography （D1.6×30 cm）. The result of element analysis showed that the contents of C and H were 37.81% and 6.737% respectively in HPS- Ⅱ, and no S, N and P. The average molecular weight of HPS- Ⅱ was 4.61×10^5. It was detected by Sepharose CL-4B column chromatography （D1.6×30 cm） with Dextran polysaccharides as standards. Infrared spectra, ^1H NMR and ^13 NMR spectroscopy indicated that HPS-Ⅱ consisted of （1→4）-α-D-Glcp backbone with（1→6）-α-D-Glcp branches.

关 键 词：真姬菇 多糖 结构分析 

分 类 号：O657[理学—分析化学] O433.4[理学—化学]