Investigation of photodynamic therapy on streptococcus mutans of oral biofilm  

作　　者：邹朝晖 高平 阴慧娟 李迎新 

机构地区：[1]Heping District of Tianjin Laser Medicine Laboratory,Biomedical Engineering Department of Tianjin Medical University [2]Heping District of Tianjin,Dental Hospital of Tianjin Medical University [3]Heping District of Tianjin Dental Hospital of Tianjin Medical University

出　　处：《Chinese Optics Letters》2008年第12期947-949,共3页中国光学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.60678047);the Science Foundation of Tianjin(No.05YFJZJC02300)

摘　　要：We investigated the effect of photodynamic therapy （PDT） with hematoporphyrin monomethyl ether （HMME） on the viability of Streptococcus mutans （S. mutans） cells on biofilms in vitro. Streptococcus mutans is the primary etiological agent of human dental caries. Since dental caries are localized infections, such plaque-related diseases would be well suited to PDT. The diode laser used in this study had the wavelength of 635 nm, whose output power was 10 mW and the energy density was 12.74 J/cm^2. HMME was used as photosensitizer. Samples were prepared and divided into five groups： （1） HMME; （2） Laser; （3） HMME＋Laser; （4） Control group （＋） with chlorhexidine; and （5） Control group （-） with sterile physiological saline. Inoculum of S. mutans incubated with HMME also examined with fluorescence microscopy. PDT exhibited a significantly （P 〈 0.05） increased antimicrobial potential compared with 20 μm/mL HMME only, laser only, 0.05% chlorhexidine, and 0.9% sterile physiological saline, which reduced the S. mutans of the biofilm most effectively. Laser and 0.05% chlorhexidine were caused reduction in the viable counts of S. mutans significantly different （P 〈 0.05） also, but these two test treatments did not statistically differ from each other. HMME group did not statistically differ with negative control group. Fluorescence microscopy indicated that HMME localized primarily in the S. mutans of the biofilm. It was demonstrated that HMME-mediated PDT was efficient at killing S. mutans of biofilms and a useful approach in the treatment of dental plaque-related diseases.We investigated the effect of photodynamic therapy （PDT） with hematoporphyrin monomethyl ether （HMME） on the viability of Streptococcus mutans （S. mutans） cells on biofilms in vitro. Streptococcus mutans is the primary etiological agent of human dental caries. Since dental caries are localized infections, such plaque-related diseases would be well suited to PDT. The diode laser used in this study had the wavelength of 635 nm, whose output power was 10 mW and the energy density was 12.74 J/cm^2. HMME was used as photosensitizer. Samples were prepared and divided into five groups： （1） HMME; （2） Laser; （3） HMME＋Laser; （4） Control group （＋） with chlorhexidine; and （5） Control group （-） with sterile physiological saline. Inoculum of S. mutans incubated with HMME also examined with fluorescence microscopy. PDT exhibited a significantly （P 〈 0.05） increased antimicrobial potential compared with 20 μm/mL HMME only, laser only, 0.05% chlorhexidine, and 0.9% sterile physiological saline, which reduced the S. mutans of the biofilm most effectively. Laser and 0.05% chlorhexidine were caused reduction in the viable counts of S. mutans significantly different （P 〈 0.05） also, but these two test treatments did not statistically differ from each other. HMME group did not statistically differ with negative control group. Fluorescence microscopy indicated that HMME localized primarily in the S. mutans of the biofilm. It was demonstrated that HMME-mediated PDT was efficient at killing S. mutans of biofilms and a useful approach in the treatment of dental plaque-related diseases.

关 键 词：Biofilms BIOFILTERS ETHERS Fluorescence Fluorescence microscopy LASERS Optical materials PHOTOSENSITIZERS PLASMONS PORPHYRINS 

分 类 号：R454[医药卫生—治疗学] R781.5[医药卫生—临床医学]