激光诱变筛选木霉菌株及木聚糖酶发酵条件优化  

Selection of Trichoderma viride strains irritated by laser and optimization of the xylanase fermentation

作　　者：刘真真[1] 茅文俊[1] 朱虹[1] 朱融融[1] 孙晓宇[1] 姚思德[2] 汪世龙[1] 

机构地区：[1]同济大学生命科学与技术学院,上海200092 [2]中国科学院上海应用物理研究所,上海201800

出　　处：《辐射研究与辐射工艺学报》2008年第6期335-342,共8页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30570376);上海市教委科技创新项目(08ZZ21);基础重点项目(06JC14068)资助

摘　　要：本实验利用激光这一高效诱变手段,对野生菌株绿色木霉Tr-02进行辐照诱变,通过两轮的定向筛选,最终得到一株高产木聚糖酶的菌株。对突变株进行发酵条件优化,结果表明,以0.5%麸皮为碳源,0.5%蛋白胨为氮源,0.1% Tween-80作为产酶促进剂,培养基初始pH值为5.0,瓶装量100mL,接种两环,28℃,180r/min培养48h,木聚糖酶活力达到281.21U·mL-1,较之相同条件出发菌株木聚糖酶活力增长31.83%。A mutation of wild strain Trichoderma Viride Tr-02 irradiated by laser was studied in this paper. A high xylanase-producing strain was obtained by two steps oriental screening finally. The results show that an optimization condition of the xylanase fermentation is as following： 0.5% corncob as the carbon source, 0.5% peptone as the nitrogen source, 0.1% Tween-80 as a enzyme-promoting agent, initial medium pH to 5.0, medium volume to 100mL, vaccinated two rings of spores, 28℃, 180 r/min, fermented 48h. The xylanase activity of the mutant reaches 281.21U/mL and is 31.83% higher than that of the original strain under the same conditions.

关 键 词：激光 绿色木霉 木聚糖酶 发酵 

分 类 号：Q631[生物学—生物物理学]