气体鼓泡强化超滤分离木聚糖酶和β-木糖苷酶  

Separation of xylanases from β-xylosidase by gas-sparged ultrafiltration

作　　者：江华[1] 

机构地区：[1]南京林业大学化学工程学院,南京210037

出　　处：《膜科学与技术》2008年第6期63-68,共6页Membrane Science and Technology

基　　金：南京林业大学科技创新基金(163030002);江苏省高校自然科学研究项目(05KJB220051)

摘　　要：研究了木聚糖酶和β-木糖苷酶的超滤分离过程中，气体鼓泡的作用、气体流量、液相流量和操作压强对溶剂透过率和分离因数的影响．结果发现，通入少量的空气即能显著改善溶剂透过率和分离因数，这种改善效果与操作条件相关，液相进料速度较低、操作压强较高时，气体鼓泡所产生的效果较为明显．在实验条件下，空气的通入可使溶剂透过率增加近60％，分离因数提高了9倍左右，增加到了36倍．适宜的空气流量为25～50mL／min；在气体鼓泡的情况下，溶剂透过率随操作压强线性地增加，而分离因数不受操作压强影响．The effects of gas sparging, gas flow rate, liquid flow rate and transmembrane pressure on the solvent flux and separation factor were examined during ultrafiltration of xylanases. Experimental results showed that gas sparging could increase permeate flux and improve the efficiency of fractionation during the ultrafiltraion of xylanases. The lower the liquid flow rate, or the higher transmembrane pressure, the more pronounced the enhancement in the ultrafiltration efficency. Gas sparging was found to increase the permeability of solvent by approximately 60 %, and the separation factor increased 9 fold and came up to 36 by gas sparging. The optimal gas flow rate was from 25 to 50 mL/min. The solvent flux increased linearly with transmembrane pressure and the separation factor was almost constant under experimental conditions with gas sparging.

关 键 词：木聚糖酶 Β-木糖苷酶 超滤 气体鼓泡 

分 类 号：TQ925[轻工技术与工程—发酵工程]