Neuropilin-1^＋ T细胞与CD4^＋ CD25^＋调节性T细胞的比较  

Comparison between Neuropilin-1^+T cells and CD4^+CD25^+ regulatory T cells

作　　者：裴向克[1] 蔡明[1] 周鸿敏[1] 刘畅[1] 徐利军[2] 陈忠华 石炳毅[1] 

机构地区：[1]解放军总医院第二附属医院全军器官移植中心,北京100091 [2]华中科技大学同济医学院附属同济医院器官移植研究所,湖北武汉430030

出　　处：《细胞与分子免疫学杂志》2008年第12期1154-1156,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(C0302050104);全军医学“十一五”计划科技攻关课题资助项目(06G115)

摘　　要：目的:分析Neuropilin-1+T细胞(Nrp-1+T细胞)与经典CD4+CD25+调节性T细胞(Treg)的关系并比较二者的免疫调节作用。方法:流式细胞术分析BALB/c小鼠脾脏T细胞上Nrp-1与CD4、CD25的表达关系并分选Nrp-1+T细胞及CD4+CD25+Treg,通过B16-F10-luc-G5黑色素肿瘤细胞体外培养实验并利用萤光成像系统,观察比较两种细胞对NK细胞杀伤B16-F10-luc-G5黑色素瘤细胞的影响。结果:CD4+CD25+Treg中表达Nrp-1的比例为(27.28±1.17)%,明显高于CD4+CD25-T细胞的(1.63±0.08)%(P<0.01);在体外实验中,Nrp-1+T细胞与CD4+CD25+Treg均能抑制NK细胞杀伤B16-F10-luc-G5黑色素瘤细胞,Nrp-1+T细胞组的肿瘤细胞数目在6、24、48、72h分别为984±15、1015±14、1261±21、1323±38,高于CD4+CD25+Treg组的931±4、983±8、1201±18、1256±18,两组肿瘤细胞数目在各时间点均有统计学意义(P<0.01)。结论:经典CD4+CD25+Treg中表达Nrp-1的细胞比例较高,Nrp-1+T细胞有负性免疫调节作用,抑制功能比CD4+CD25+Treg更强,可以作为一类新的Treg亚群。AIM： To analyze the relationship between Nrp-1（ neuropilin-1） positive T cells（ Nrp-1^＋ T cells） and CD4^＋ CD25^＋ regulatory T cells （CD4^＋ CD25^＋ Treg ） and compare their immunoregulatory effects. METHODS： The expression of Nrp-1, CD4 and CD25 on the splenic T cells of BALB/c mice was detected by flow cytometry and Nrp-1^＋ T cells and CD4^＋ CD25^＋ Treg were sorted. Then their effects of on NK cells killing B16-F10-luc-G5 melanoma cells were compared in vitro by bioluminescence imaging system. RESULTS： The expression of Nrp-1 on CD4 ^＋ CD25^＋ Treg was （27.28±1.17）%, which was significantly higher than that （1.63±0.08）% on CD4^＋CD25^- Tcells（P〈0.01）. Both Nrp-1^＋ T cells and CD4^＋ CD25^＋ Treg inhibited the effect of killing NK cells on B16-F10-luc-G5 melanoma cells in vitro. At 6, 24, 48 and 72 h, the number of tumor cells in Nrp-1^＋ T cell group was higher than that in CD4^＋ CD25^＋ Treg group （984± 15 vs 931±4, 1015±14 vs 983±8, 1261±21 vs 1201±18 and 1323±38 vs 1256±18, respectively）. There was significant statistical difference between the two groups at each time point（P〈0.01 ）. CONCLUSION： The proportion of CD4^＋ CD25^＋ Treg expressing Nrp-1 is high. Nrp-1^＋ T cells show negtive immunoregulatory effect, which is more powerful than CD4^＋ CD25^＋ Treg. Nrp-1^＋ T cells may be regarded as a new subgroup of Treg.

关 键 词：NEUROPILIN-1 调节性T细胞 萤光成像 

分 类 号：R730.3[医药卫生—肿瘤]