Stathmin/Oncoprotein18(Op18)在人脑胶质瘤中的表达研究  

Research on expression of stathmin/oncoprotein18 (Op18) gene in human brain gliomas

作　　者：王大鹏[1] 慕璐岩[1] 刘东健 王策[1] 董白晶[1] 王雪峰[1] 汤加滨[1] 胡炜[1] 刘晓谦[1] 

机构地区：[1]哈尔滨医科大学第四附属医院神经外科,黑龙江哈尔滨150001 [2]红兴隆管局中心医院神经外科,黑龙江双鸭山155811

出　　处：《中华神经外科疾病研究杂志》2008年第6期517-520,共4页Chinese Journal of Neurosurgical Disease Research

摘　　要：目的探讨Stathmin/oncoprotein18(Op18)基因在人脑胶质瘤中的表达规律及意义。方法分别采用免疫组化法和Western blot法检测10例正常脑组织和56例脑胶质瘤中Stathmin蛋白的表达。结果免疫组化法检测Stathmin蛋白在正常脑组织、低级别胶质瘤(Ⅰ～Ⅱ级)及高级别胶质瘤(Ⅲ～Ⅳ级)中阳性表达率分别为20%、65%、100%。正常脑组织分别与Ⅰ～Ⅱ级组、Ⅲ～Ⅳ级组比较,均有显著性差异(P<0.05);Ⅰ～Ⅱ级组与Ⅲ～Ⅳ级组比较,有显著性差异(P<0.05)。Western blot法检测显示,Stathmin蛋白在胶质瘤中的表达明显增高,正常脑组织分别与Ⅰ～Ⅱ级组、Ⅲ～Ⅳ级组比较,差异均有显著性(P<0.01),Ⅰ～Ⅱ级组与Ⅲ～Ⅳ级组比较,差异有显著性(P<0.01)。结论Stathmin在脑胶质瘤中过表达,Stathmin可能为脑胶质瘤的生物治疗提供一个新靶点。Objective To investigate the expression of Stathmin/oncoprotein 18 （Op18） in human brain gliomas and its significance. Methods Fifty-six cases of human brain gliomas were examined by immunohistochemical staining and Western blot method （including 10 cases of normal cerebral tissues）. Results The immunohistochemical analysis demonstrated that the positive rate of Stathmin in normal human brain tissues,grade Ⅰ -Ⅱ gliomas and grade Ⅲ - Ⅳ gliomas were 20%, 65% and 100% respectively. There was significant difference between normal human brain tissues and grade Ⅰ -Ⅱ glioma, grade Ⅲ - Ⅳ （ P 〈 0. 05 ） respectively ; the difference between Ⅰ -Ⅱ grade glioma and Ⅲ -Ⅳ grade glioma was significant （P 〈 0.05）. The expression level of stathmin in the gliomas was higher than that of normal brain tissues by Western blot method. There were significant difference between normal brain tissues and grade Ⅰ -Ⅱ glioma, grade Ⅲ - Ⅳ glioma （P 〈0. 01 ） respectively; the differences between grade Ⅰ -Ⅱ glioma and grade Ⅲ -Ⅳ glioma were significant （P 〈 0. 01 ）. Conclusion Stathmin overexpresses in human brain gliomas which may be a new biotherapy target in the treatment of brain gliomas.

关 键 词：脑胶质瘤 STATHMIN 免疫组化法 WESTERN BLOT 

分 类 号：R739.4[医药卫生—肿瘤]