Survivin反义核酸对大肠癌HT-29细胞侵袭力的影响  

Effects of Survivin antisense oligodeoxynucleotides on invasive ability of HT-29 colorectal carcinoma cells

作　　者：宫向前[1] 邢建华[1] 李刚[1] 李玉华[1] 丁印鲁[2] 寿楠海[2] 

机构地区：[1]青岛大学附属烟台毓璜顶医院,烟台264000 [2]山东大学齐鲁医院普外科

出　　处：《实用肿瘤学杂志》2008年第6期501-503,共3页Practical Oncology Journal

摘　　要：目的观察反义Survivin寡核苷酸对人大肠癌HT-29细胞侵袭力的影响。方法应用针对Survivin模板序列的反义寡核苷酸链处理大肠癌HT-29细胞,观察细胞培养生长增殖状况,半定量RT-PCR检测不同组别Survivin基因表达的水平,应用Transwell小室检测体外侵袭力变化。结果反义组细胞数量少,生长稀疏,细胞贴壁缓慢;大肠癌细胞的集落形成能力受到抑制,经过RT-PCR扩增后半定量分析结果显示,反义核酸治疗组的表达量低于对照组和正义组。侵过小室滤膜细胞数减小。结论Survivin反义寡核苷酸作用后的大肠癌HT-29细胞株生长受到抑制,细胞株侵袭力降低。Objective To investigate the effect of Survivin gene on the growth of human colorectal canc- er HT - 29 cells and to explore the feasibility of gene therapy in human co!orectal carcinoma. Methods The hu- man colorectal cancer HT - 29 cell line was treated with ASODNs. The effects of Survivin - ASODN on the growth of HT - 29 were studied by cell colony assay, reverse transcription polymerse chain reaction. The invasive ability was measured by number of cells to penetrate polycarbanates coated with matrigel. Results The number of HT - 29 cells colony was inhibited after the cells were treated for 48 hours. The cell proliferation was inhibited effec- tively, too. The expression of Survivin mRNA decreased sharply in HT -29 cells treated with ASODNs. The cells number that penetrated polyearbonates was significantly lower in the ASODNs group than that of control group. Conclusions The antisense oligodeoxynucleotides were capable of inhibiting the proliferation and the invasive ability of human colorectal carcinoma HT -29 ceils.

关 键 词：大肠肿瘤 细胞系 SURVIVIN 反义寡核苷酸 

分 类 号：R735.34[医药卫生—肿瘤]