槟榔提取物体外抗柯萨奇病毒B3及单纯疱疹病毒1型和乙型肝炎病毒的研究  

Investigation on Antiviral Effect of Areca catechu L.Extract in vitro

作　　者：王小燕[1] 张美英[1] 王亚峰[1] 郝静[1] 刘秋英[1] 王一飞[1] 张颖君[2] 杨崇仁[2] 

机构地区：[1]广州暨南大学医药研究开发基地,广东广州510632 [2]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650204

出　　处：《时珍国医国药》2008年第12期2954-2955,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金.广东省人民政府自然科学联合基金(No.U0632010)

摘　　要：目的从槟榔9种提取物中体外筛选出抗柯萨奇病毒B3,单纯疱疹病毒1型活性成分,从其中3种提取物中体外筛选出抗乙型肝炎病毒的活性成分。方法通过观察药物毒性、病毒引起的细胞病变效应、MTT法,判断药物毒性及药物抗病毒效应。结果槟榔9种提取物对Hela,Vero细胞具有一定的毒性,而无抗病毒活性,3种提取物不能有效地抑制HepG2.2.15细胞HBeAg的表达。结论槟榔9种提取物体外无抗柯萨奇病毒B3、单纯疱疹病毒1型的作用,3种提取物体外无抗乙型肝炎病毒的作用。Objective To screen nine extracts of Areca catechu L. for anti - Coxackievirus B3, HSV - 1 and three extracts of Areca catechu L. for anti - HBV in vitro. Methods The toxic and antiviral effect was evaluated by observing toxic effect , cytopathic effect （ CPE ） , MTT colorimetric assay for vital cell rate. Results Nine extracts had certain toxic effects on Hela and Vero cells in vitro, but they had no antiviral activities. Three extracts of Areca catechu L. could not inhibit the expression of HepG2.2.15 cell g HBeAg . Conclusion Nine extracts of Areca catechu L. have no effect of anti - Coxackievirus B3, HSV - 1 and three extracts of Areca catechu L. have no anti - HBV in vitro.

关 键 词：槟榔 柯萨奇病毒 单纯疱疹病毒1型 乙型肝炎病毒 

分 类 号：R285.5[医药卫生—中药学]