新型磁性纳米粒子固定脂肪酶的研究  

Study on the Immobilization of Lipase with Novel Magnetic Nanoparticles

作　　者：王燕佳 蒋惠亮 方银军 任国晓 

机构地区：[1]江南大学化学与材料工程学院,江苏无锡214122 [2]浙江赞宇科技有限公司,浙江杭州310009

出　　处：《食品与生物技术学报》2008年第6期98-102,共5页Journal of Food Science and Biotechnology

基　　金：浙江省科技攻关项目(021101047)

摘　　要：用无皂乳液聚合制备了P(AM-co-St)-Gb(Ⅲ)磁性高分子纳米微球,在此基础上通过共价键合固定脂肪酶。结果表明:固定脂肪酶后的磁性纳米微球具有优异的磁分离能力;钆离子对固定化酶有明显的激活作用,当钆离子质量分数为1.4%时,偶联率和活力回收率分别提高88%和71%;脂肪酶被固定化后其pH值稳定性、操作稳定性均比自由酶明显提高。Based on the P（AM-co-St）-Gb（ Ⅲ ） magnetic nanoparticles by emulsifier free, emulsion, lipase was immobilized on this nanoparticles with covalent linkage. It was found that immobilized lipase magnetic nanoparticles possessed excellent ability of magnetic separation. Gd（Ⅲ） could activate the immobilized lipase, when the weight ratio of Gd（Ⅲ） is 1.4%, the coupled yield and activity of the immobilized Lipase was increased by 88 % and 71%, respectively. As compared with free lipase, the pH and stabilities of immobilized lipase were increased remarkly.

关 键 词：磁性纳米粒子 钆 固定 脂肪酶 

分 类 号：TQ925.6[轻工技术与工程—发酵工程]