人纤溶酶原kringle 5在大肠杆菌中克隆和分泌表达  

Cloning and Secretary Expression of Human Plasminogen Kringle 5 in E.coli

作　　者：张宇[1] 张名姝[2] 李雅雯[1] 郑权[1] 

机构地区：[1]北京航空航天大学生物与医学工程学院,北京100083 [2]中国科学院生物物理研究所,北京100101

出　　处：《生物技术通报》2008年第6期132-134,共3页Biotechnology Bulletin

基　　金：北京航空航天大学首届大学生科研训练计划基金(4010100)

摘　　要：构建能够表达分泌性的人纤溶酶原kringle 5(简称hPK-5)的大肠杆菌工程菌。用PCR方法扩增获得hPK-5基因,构建原核表达载体pET-22b(+)/hPK-5,转化大肠杆菌BL21(DE3),经IPTG诱导表达并鉴定其免疫学活性。成功表达14kD的重组hPK-5,且约占菌体分泌性蛋白20%以上,经Western Blot分析表明其具有hPK-5的免疫抗原活性。人纤溶酶原kringle 5在大肠杆菌中BL21(DE3)获得可分泌表达。The aim is to establish an engineering E.coli strain which could produce secretory human plasminogen kringle 5（hPK-5）.hPK-5 gene was amplified by PCR and the prokaryotic expression vector pET22b（＋）/hPK-5 was constructed.Recombinant hPK-5 protein was expressed by inducing E.coli BL21（DE3）and the immunological activity of which was identified by western blot.The recombinant hPK-5 about 14kD was expressed successfully in soluble form,consisting of 20% of the secretary bacterial proteins by SDS-PAGE.Immunological analysis indicated that the protein could react with antibodies against hPK-5.Thus,it proved that human plasminogen kringle 5 can be expressed by engineering E.coli strain BL21（DE3）.

关 键 词：人纤溶酶原Kringle 5 大肠杆菌 克隆 分泌性表达 

分 类 号：Q78[生物学—分子生物学]