N^+注入诱变选育β-葡萄糖苷酶高产菌株及其产酶条件的优化  

Breeding of β-Glucosidase-producing Strains by N^+ Implantation and Research on Fermentation Conditions

作　　者：沈加成[1] 朱明田[1] 曲音波[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,山东济南250100

出　　处：《纤维素科学与技术》2008年第4期7-11,共5页Journal of Cellulose Science and Technology

摘　　要：以筛选得到能够有效利用木糖渣作为碳源生产β-葡萄糖苷酶的菌株为目的,利用N+注入棘孢曲霉L22,筛选得到一株β-葡萄糖苷酶活提高1倍的突变株NIP35。通过单因子实验结合正交实验,对其培养基组成如碳源、氮源、表面活性剂等进行了优化,使其产酶水平最终提高了近2倍。NIP35, the β-glucosidase high-yield strain, was obtained through N＋ implanting into Aspergillus aculeatus L22. The yield increased 1 fold than that of the original. The culturing conditions such as carbon source, nitrogen source, and surfactant effect on fermentation of NIP35 were studied. Under the optimum condition, the yield of β-glucosidase increased nearly 2 folds.

关 键 词：Β-葡萄糖苷酶 N^+注入 棘孢曲霉 木糖渣 

分 类 号：Q556.2[生物学—生物化学]