广东地区宫颈病变HPV81型L1基因多态性分析  

Polymorphisms of human papillomavirus type 81 L1 gene in patients with cervical lesions in Guangdong

作　　者：曾莉[1] 俞守义[1] 胡朝晖[2] 章俊[3] 

机构地区：[1]南方医科大学公共卫生与热带医学学院．广东广州510515 [2]广州金域医学检验中心,广东广州510182 [3]南方医科大学珠江医院肾内科,广东广州510280

出　　处：《南方医科大学学报》2008年第12期2210-2212,共3页Journal of Southern Medical University

基　　金：广东省医学科研基金(A2007361)

摘　　要：目的调查我国广东地区宫颈病变患者组织中HPV81型的阳性率及其L1基因多态性。方法采用HPV通用引物对1200例HPVDNA检测阳性的宫颈脱落细胞标本中的HPVL1区基因序列进行PCR扩增,并结合基因测序分析。结果和结论获得HPV81型4例,检出率为0.33%。与Genbank标准株相比,广东地区发现的HPV81L1基因有多处不同,与日本发现的HPV81病毒株相近。Objective To investigate the positivity rates and genetic polymorphism of human papillomavirus （HPV） type 81 （HPV81） L1 gene in patients with cervical cancer in Guangdong. Method HPV L1 genes fragment in 1200 positive cervical samples detected with HC-Ⅱ for high-risk HPV DNA were amplified by HPV-specific PCR with consensus primers, and the L1 genes of HPV81 were sequenced for mutation analysis. Results Four HPV81-positive cases were found in the total of 1200 cases for the positivity rate of 0.33%. Point mutations of HPV81 L1 gene were found in all of the samples. Conclusion Compared with the HPV81 prototypes published in GenBank, several new point mutations are found in the L1 genes of the HPV81 in Guangdong, and these mutations are close to those found in Japan.

关 键 词：人类乳头瘤病毒 宫颈肿瘤 L1基因 基因多态性 

分 类 号：R737.33[医药卫生—肿瘤]