L-异亮氨酸产生菌的定向育种  

Rational Breeding of an L-isoleucine Producer

作　　者：来彩霞[1] 陈炜[1] 刘党生[1] 王宏 

机构地区：[1]沈阳药科大学药物研究所,沈阳11015

出　　处：《沈阳药科大学学报》1998年第1期47-50,共4页Journal of Shenyang Pharmaceutical University

摘　　要：以Ｌ－异亮氨酸产生菌Ⅳ ６９为出发菌株，采用紫外线诱变处理，从以缬氨酸为唯一碳源，唯一氮源和唯一碳、氮源的培养基上挑取生长迅速的菌落，筛选缬氨酸分解代谢性较高的菌株，以达到保持主产物产量，降低杂酸含量的目的．由以缬氨酸为唯一碳、氮源培养基上筛选到的突变菌株 ＣＮ５９ 缬氨酸产量由 ７．３ ｇ·Ｌ－１下降到１４ ｇ·Ｌ－１，而异亮氨酸产量无变化．An L-isoleucine producer Ⅳ 69 was treated with UV and the mutants growing rapidly were selected from the media in which L-valine was as the sole carbon source,the sole nitrogen source as well as the sole carbon and nitrogen source in order to get the strains of high activity in decomposing L-valine and lower the L-valine amount in broth with L-isoleucine amount keeping relatively stable.The strain CN59 was selected from the medium in which L-valine was as the sole carbon and nitrogen source and its L-valine amount decreased from 7.3 g·L^-1 to l.4 g·L^-1.

关 键 词：异亮氨酸 菌种选育 发酵 定向育种 

分 类 号：TQ464.7[化学工程—制药化工] TQ922[轻工技术与工程—发酵工程]