AsiaⅠ型口蹄疫病毒灭活疫苗毒株不同宿主系传代中VP1基因的遗传变异研究  

Studies on Genetic Variation of VP1 Genes of AsiaⅠ FMDV Inactivated Vaccine Strains from Serial Passages in Different Hosts

作　　者：朱晶晶[1,2] 盛卓君 符子华[1] 易忠[1] 魏玉荣[1] 黄炯[1] 马文革[1] 胡俊 朱刚 徐亚萍 

机构地区：[1]新疆畜牧科学院兽医研究所,乌鲁木齐830000 [2]新疆农业大学,乌鲁木齐830052 [3]新疆动物防疫监督总站,乌鲁木齐830000 [4]新疆天康畜牧生物技术股份有限公司,乌鲁木齐830032

出　　处：《中国畜牧兽医》2008年第12期65-70,共6页China Animal Husbandry & Veterinary Medicine

基　　金：新疆维吾尔自治区高技术研究项目(200511101)

摘　　要：口蹄疫病毒结构蛋白VP1参与构成病毒粒子的主要中和抗原位点,是4种结构蛋白中最易发生变异的。在病毒传代过程中,对VP1基因进行遗传变异分析是口蹄疫疫苗研制中不可或缺的环节。为此,作者扩增了经不同宿主系（乳鼠、BHK21细胞）连传不同代次的AsiaⅠ型毒株的VP1基因,并对其进行遗传变异分析,毒株间核苷酸同源性为99.4%-99.8%,推导氨基酸序列同源性为98.6%-100%;制苗毒株经过不同宿主系有限传代后,与传代前的原毒（MF1）相比,VP1基因未发生大的变异,主要抗原位点较稳定,说明以此种方式获得的制苗毒株制备的灭活疫苗是稳定的,适用于该毒株流行区域内相关家畜的免疫预防。The VP1 gene of FMDV contains major antigenic determinant and has the highest mutation rate in four structural proteins. Analysis of genetic variation during the passage is indispensable for vaecine research. This paper was studied on the sequence analysis of the VP1 gene of inactivated vaccine strains of FMDV which was generated in different hosts such as suckling mouse and BHK21 cell. The result demonstrated that the homologies among VP1 gene sequences were 99.4% to 99.8%, and the homologies among the deduced amino acid sequences were 98.6% to 100%. Compared with the MF1, VP1 gene and the main antigenic sites were conserved. So it is stable for producing inactivated vaccine and suitable to immune prevention of related livestock in prevailed area.

关 键 词：FMDV ASIAI型 灭活疫苗毒株 VP1基因 遗传变异分析 

分 类 号：S852.659.6[农业科学—基础兽医学]