不同固定化菊粉酶方法的比较及条件优化  

Comparison and Optimization of Immobilization Methods of Inulinase

作　　者：苏豫梅[1] 李清清[1] 李秉超[1] 孙忠思[1] 魏忠环[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110161

出　　处：《现代食品科技》2008年第12期1296-1299,共4页Modern Food Science and Technology

摘　　要：采用聚乙烯醇-海藻酸钙(PVA-CA)凝胶包埋法、壳聚糖交联法、D201-GM阴离子大孔树脂吸附交联法及人造棉绒布共价偶联法对菊粉酶进行固定化。通过对四种方法的对比得出最佳固定化方法为D201-GM阴离子大孔树脂吸附交联法,其固定化酶酶活力、酶活力回收率及操作稳定性均优于其它三种方法。以D201-GM阴离子大孔树脂为载体,对影响菊粉酶固定化的重要因素进行了考察,获得最佳固定化条件。实验结果表明,在pH为5.4,载体与酶的比例为1:2的条件下,振荡吸附3h后,加入0.5%(终浓度)戊二醛,15℃交联4h时即可获得较好的固定化效果。Four methods for inulinase immobilization, including PVA-CA gelatin entrapment, chitosan crosslinking, D201 macroporous resin absorption-crosslinking method and cotton cloth eovalent coupling method, were compared and D201 macroporous resin absorption-crosslinking method was shown to be the best due to its highest enzyme activity, recovery of enzyme activity and operational stability of the enzyme. The optimal immobilized conditions using D201 rnacroporousas as the carrier were determined as follows： pH of 5.4, ratio of carrier to enzyme of1：2, absorption time with shaking of 3 h, glutaraldehyde dosage of 0.5% （after absorption）, the cross linking temperature of15℃ and crosslinking time of 4 h.

关 键 词：菊粉酶 固定化 D201-GM阴离子大孔树脂 

分 类 号：Q814.2[生物学—生物工程]