大豆磷脂组成的薄层色谱分析  

Analysis of Soybean Phospholipids by Thin-Layer Chromatography

作　　者：郭勍[1] 徐桂云[1] 常理文[1] 

机构地区：[1]中国科学院化学研究所,北京100080

出　　处：《分析化学》1998年第1期81-84,共4页Chinese Journal of Analytical Chemistry

摘　　要：采用国产硅胶板和二次展开的薄层色谱法，对具有重要的生物功能的大豆磷脂组成进行了分析，获得了大豆磷脂中１１个重要组分的定性结果。用已知样品量的薄层光密度扫描方法建立了卵磷脂定量方法，线性关系良好，并用于实际样品的测定，获得了满意结果。Eleven components in soybean phospholipids were separated by TLC, using silica gel plate made in China and twice development. The quantitative analysis has been achived with dual wavelength TLC scanning. The calibration curve for PC is in the range between 0. 2-5. 0 μg and the detection limit is 0. 2 ×10- 6 g. This method has been applied to the analysis of PC in lecithin.

关 键 词：大豆磷脂 薄层色谱 卵磷脂 磷脂 

分 类 号：Q545.103[生物学—生物化学]