应用测试片快速检测食品中金黄色葡萄球菌的研究  

STUDY ON QUICK DETECTION OF STAPH.AUREUS IN FOOD BY DETECTIVE SLIP

作　　者：许如苏[1] 林彩华[1] 孙霞 亢卫民[1] 马少玲[1] 杨梓坚[1] 陈冠武[1] 卢新 

机构地区：[1]汕头出入境检验检疫局,广东汕头515031 [2]广州绿洲生化研究中心,广东广州510663

出　　处：《食品研究与开发》2009年第1期94-97,共4页Food Research and Development

基　　金：广东省科学技术厅农业攻关计划(2006B20501011)

摘　　要：采用法国进口的金黄色葡萄球菌显色培养基为主要原料,运用微生物测试片专有技术将其制成一次性的快速测试片。经测试,该测试片的检测灵敏度高,特异性强,重复性好。其对纯菌的检测下限可达3cfu/mL,与营养琼脂计数、Baird-Parker培养基计数和显色培养基计数比较,检测结果均无显著性差异(P>0.05)。对染菌样品的检测结果,测试片与3MTM Petrifilm法无显著性差异(P>0.05),而检测下限明显低于GB标准方法。A kind of Staph.aureus detective slip, which was made of CHROM agarTM Staph.aureus by technicality technique of detective slip for microorganism was studied. The results showed that the detective slip method was a higher sensitivity, stronger specificity, better repeatablity and rapid method. It could detect 3 cfu/mL Staph.aureus in pure culture and the results detected by the detective slips were not significant different from that by nutrient agar or Baird-Parker agar or CHROM agar^TM Staph.aureus （P〉0.05）. The results of samples arti- ficially contaminated with Staph.aureus detected by detective slips were consistent with those by 3M^TM Petrifilm. The lowest detectable limit of detective slip was significant lower than that of GB standard.

关 键 词：金黄色葡萄球菌 快速测试片 食品 

分 类 号：R155.5[医药卫生—营养与食品卫生学]