丙型肝炎病毒非结构基因5b区新发现的一个移码突变  

A NOVEL FRAME-SHIFT MUTATION FOUND IN NONSTRUCTURAL 5b REGION OF HEPATITISC VIRUS

作　　者：魏来[1] 潘修成 吴文漪 王宇[1] 陶其敏[1] 

机构地区：[1]北京医科大学肝病研究所

出　　处：《徐州医学院学报》1998年第2期87-89,共3页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金;江苏省委333工程基金;江苏省教委自然科学基金

摘　　要：目的分析丙型肝炎病毒（ＨＣＶ）非结构基因５ｂ（ＮＳ５ｂ）区部分核苷酸序列特征。方法以逆转录巢式聚合酶链反应获得位于７９１８位至８２９３位间的ｃＤＮＡ片段，克隆后测定重组子序列，计算机分析其特征。结果获得了３７６碱基对长的ＨＣＶＮＳ５ｂ基因（ＨＣ－Ｐ３９），与ＨＣＶ－１，ＨＣ－Ｃ２，ＨＣＶ－Ｊ，ＨＣＶ－ＢＫ，ＨＣＶ－Ｊ６及ＨＣ－Ｊ８相比，核苷酸水平的同源性分别为７６．９７％，８８．９２％，８７．７５％，８９．２１％，６３．８５％及６４．４３％。在核苷酸８１１１位至核苷酸８１２４位发现一个新的移码突变，并因此在核苷酸８１２２位形成一个终止突变。结论在ＨＣＶＮＳ５ｂ区发现一个新的移码突变。Objective To analyse the nucleotide sequence of nonstructural 5b genome (NS5b) of hepatitis C virus (HCV). Methods The cDNA segment of HCV ranged from position 7918 to position 8293 was obtained by reverse transcription nested polymerase chain reaction (RT-PCR) , and was then cloned and sequenced. Results The genome obtained, containing 376 bp and corresponding to HCV NS5b, was designated as HC-P39. It was found that HC-P39 shared 76.97%,88.92%,87.75%,89.21%,63.85% and 64.43% nucleotide homology with HCV-1, HC-C2, HCV-J, HCV-BK, HC-J6 and HC-J8 respectively, and hence belonged to genotype Ⅱ/1b. Comparison of HC-P39 with the 6 reported genotypes revealed there was a novel frame-shift mutation between position 8111 and position 8124, which was ended by the introduction of an in-frame stop codon beginning from position 8122. Conclusion A novel frame-shift mutation in HCV NS5b genome was recognized through the study.

关 键 词：丙型肝炎病毒 序列分析 基因突变 移码突变 

分 类 号：R512.63[医药卫生—内科学] R373.21[医药卫生—临床医学]