千代田草莓乙烯受体Etr2基因克隆及序列分析  

Cloning and Sequence Analysis of a DNA Encoding Ethylene Receptor Etr2 from Chiyoda Strawberry

作　　者：宋春丽[1] 马俊莲[2] 唐霞[2] 张子德[2] 刘永巨[2] 

机构地区：[1]河北农业大学中兽医学院,河北定州073000 [2]河北农业大学食品科技学院,河北保定071001

出　　处：《华北农学报》2008年第6期46-49,共4页Acta Agriculturae Boreali-Sinica

基　　金：河北省科技攻关项目(04395501D-3)

摘　　要：克隆了与草莓成熟有关的乙稀受体FaEtr2基因片段,为进一步研究FaEtr2基因功能并通过基因技术改善草莓贮运性能奠定基础。以千代田草莓成熟果实中分离到的基因组DNA为模板,经PCR扩增到1条约1.0 kb的特异片段,将该片段克隆到pGEM-T easy vector上经测序分析,基全长共1 049 bp,编号349个氨基酸残基。序列分析结果表明,该序列与Chandler-Etr2的cDNA序列同源性99%、氨基酸序列同源性为98%。In this study a ripening related ethylene receptor etr2 gene in strawberry fruits was cloned for the following-up research on gene function and improving varieties of strawberry through transgenic technology. A pair of primers were designed and synthesized based on the sequence reported of Chandler-Err2. Using genome DNA extracted from Chinyoda strawberry mature fruits as template the specific PCR product of etr2 was obtained, then it was ligated to pGEM- T easy vector and sequenced. The sequencing data showed that the PCR product was 1 049 bp encoding 349 predicted amino acid residues. Comparison with the cDNA sequence from Chandler-etr2 indicated the horology was 99 %, and amino acid identities were 98 %.

关 键 词：草莓 乙烯受体 基因克隆 序列分析 

分 类 号：S668.4[农业科学—果树学]