端粒酶催化亚基hTERT调控Hela细胞周期的实验研究  

Regulation of hTERT on the expression of cell cycle gene in Hela cell line in vitro

作　　者：李相武 周雅彦[2] 朱俊祎[2] 李丹[2] 

机构地区：[1]湖南省长沙市芙蓉区疾病预防控制中心,410005 [2]湖南大学生命科学与技术研究院

出　　处：《公共卫生与预防医学》2008年第6期4-7,共4页Journal of Public Health and Preventive Medicine

摘　　要：目的探讨hTERT基因与细胞周期因子之间的关系,从细胞周期调控的角度研究反义hTERT影响肿瘤细胞生长的机制。方法脂质体介导将hTERT反义寡核苷酸(hTERT-ASODN)转染到Hela细胞中,运用RT-PCR方法分析hTERT-ASODN对细胞周期相关基因表达水平的改变,流式细胞仪技术检测细胞周期分布的变化。结果hTERT-ASODN可在mRNA水平封闭Hela细胞中hTERT基因的表达,并使细胞周期相关基因如CyclinE1,CyclinB1,CyclinD1的表达水平出现明显变化。流式细胞仪的检测结果显示,hTERT-ASODN转染组的细胞数在G0/G1期增多,而在S期及G2/M期减少,但无特征性的凋亡峰出现。结论hTERT基因参与调控肿瘤细胞中相关细胞周期因子的表达。Objective To investigate the effects of human telomerase reverse transcriptase （hTERT） antisense phosphomthioate oligodeoxynucleotide （ASODN） on cell growth and the relationship between the hTERT and cell cycle regulator in vitro. Methods After transfecting hTERT-ASODN into human Hela cell line with Liposome, the expression of cell cycle regulator was studied by RT-PCR. Cell cycles were detected by flow cytometry （FCM） analysis technology. Results The expression level of hTERT gene significandy decreased on mRNA level, along with cell cycle gene such as CyclinE1, CyclinB1 ,CyclinD1 changed respectively. FCM suggested that ASODN of hTERT arrest the cell cycle progression in G0/G1 phase. But no characteristic apoptosis peak was found. Conclusion It is proposed that hTERT has a novel role in the modulation of cell cycle regulator expression.

关 键 词：HTERT 反义寡核苷酸 细胞周期 HELA细胞 

分 类 号：R-33[医药卫生]