60Coγ射线辐照毛竹种子的细胞学诱变效应  

CYTOLOGICAL EFFECTS INDUCED BY ^(60)Coγ-IRRADIATION ON MOSO SEED

作　　者：王新新[1] 高健[2] 杨培浩[1] 周立人[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]国际竹藤网络中心,国家林业局竹藤科学与技术重点开放实验室,北京100102

出　　处：《核农学报》2008年第6期770-775,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家“十一五”林业科技支撑计划项目(2006BAD19B0203)

摘　　要：利用6种不同剂量的60Coγ射线辐照毛竹种子,研究γ射线辐照对毛竹种子根尖细胞有丝分裂、染色体畸变及核畸变的影响。结果表明,在一定范围内,剂量低于60Gy的辐照对毛竹根尖细胞有丝分裂有促进和刺激效应;高于90Gy对毛竹根尖细胞有丝分裂有一定的抑制作用。同时,辐照还能诱发染色体畸变和核畸变,出现染色体桥、落后染色体(团)、游离染色体(团)、染色体断片、染色体粘连、微核、小核、双核、核出芽、核耳和核裂等类型。染色体畸变率、核畸变率与剂量间存在显著的正相关,相关系数分别为0.8875、0.9982,线性方程式分别为Y=0.0392X-0.4313,Y=0.0530X-0.0783;染色体畸变率与微核率间也存在着较好的相关性,线性表达式为Y=0.8836X-0.4525,相关系数为0.8345,F=25.22>F0.01。In this experiment, Moso seeds were irradiated by different doses of 60 Coγ rays and the effects on chromosomal aberration, nuclear aberration and mitosis of the root tip ceils of were studied. The results indicated that γ-rays irradiation with lower doses could promote and stimulate mitosis of root tip cells, and with high doses could inhibit mitosis of root tip cell. Furthermore, γ-rays irradiation could induce many types of chromosomal and nuclear aberration, such as chromosome bridges, chromosome fragment, laggard chromosome （clump）, free chromosome （clump）, chromosome conglutination, micronucleus, minornucleus, double nucleus, nuclear budding, nuclear bleb, nuclear dosages and frequencies of chromosomal and nuclear aberration of root tip 0.3756, Y = 0.0530X - 0.0783, respectively. The correlation coefficient fragmentation and so on. The relationship between cells was fitted in the model of Y = 0.0390X- was 0.8934 and 0.9982. The relationship between frequency of chromosomal aberration and frequency of Moso micronucleus of root tip cell was fitted in with the model Y = 0.8836X - 0.4525. The correlation coefficient was 0.8345, F = 25.22 〉 F0.01 .

关 键 词：毛竹种子 60Coγ射线辐照 染色体畸变 核畸变 有丝分裂 

分 类 号：S795[农业科学—林木遗传育种]