y-氨基丁酸对胰腺癌细胞增殖及转移作用的实验研究  

Effects of GABA on proliferation and metastasis of pancreatic cancer cell

作　　者：刘伟 王营[2] 余胜利[2] 刘军权[4] 陈复兴[4] 费素娟[2] 滕一农[2] 王人灏 

机构地区：[1]徐州市民政医院内科,江苏221002 [2]江苏省徐州医学院附属医院消化科,江苏221002 [3]徐州市民政医院普外科,江苏221002 [4]解放军第97医院中心实验室

出　　处：《中华胰腺病杂志》2008年第6期369-371,共3页Chinese Journal of Pancreatology

基　　金：江苏省教育厅自然研究项目（04KJD320203）;江苏省徐州市科技局科技计划项目（X2004425-3）

摘　　要：目的观察y-氨基丁酸（GABA）对胰腺癌SWl990细胞增殖、细胞周期及基质金属蛋白酶MMP-2、MMPO表达的影响。方法应用不同浓度（0—320μm0L／L）GABA干预SWl990细胞，采用MTr法和流式细胞仪检测细胞增殖和细胞周期。RT．PCR检测细胞MMP-2mRNA和MMP-9mRNA的表达，Westernblot检测MMP-2和MMP-9蛋白表达。结果GABA促进胰腺癌SWl990细胞的生长，使Go／Gl细胞减少，S期和G2／M细胞增多。320μmol/L的GABA干预细胞后的A。值为1．11±0．03，较无GABA干预组的0．56±0．01显著增加（P〈0．01）；Go／G，细胞为（46．18±1．12）％，较无GABA干预组的（87．29±1．34）％显著减少（P〈0．01）；MMP-2mRNA、MMP-9mRNA及蛋白表达分别为8．6、6．8、10．5、8．4，较0—40μmoL／L组显著增加（P〈0．05）。结论GABA能促进SWl990细胞增殖和MMPs的表达。Objective To observe the effects of GABA on proliferation, cell cycle and expression of MMP-2, MMP-9 of pancreatic cancer cell line SW1990. Methods The effects of different concentration of GABA （0 - 320 μmol/L） on proliferation and cell cycle of pancreatic cancer cell line SW1990 was investigated by MTT assay and flow cytometry analysis, respectively. Expressions of MMP-2 and MMP-9 proteins were evaluated by Western blot analysis. Results GABA could promote the proliferation of SW1990 cells and influence the distribution of cell cycle, which made less cells of G0/Gl phase and more cells of S and G2/M phase. The value of A570 after GABA pretreatment at a dose of 320 p, mol/L was 1.11 ± 0.03, which was significantly higher than that in the control group （0.56 ± 0.01, P 〈 0.01 ）, the cells of Go/Gl phase was （46.18 ± 1.12 ） %, which was significantly lower than （87.29 ± 1.34） % in the control group （P 〈 0.01 ） ;the expressions of MMP-2 mRNA, MMP-9 mRNA and their proteins were 8.6, 6.8, 10.5, 8.4, respectively, which were significantly higher than those in the groups of the doses of 0 - 40 μmol/L （ P 〈 0. 05 ）. Conclusions GABA could influence the proliferation and expression of MMP of SW1990 cells.

关 键 词：胰腺肿瘤 Y-氨基丁酸 细胞增殖 细胞凋亡 基质金属蛋白酶类 

分 类 号：R735.9[医药卫生—肿瘤]