氨氯地平对氧化型低密度脂蛋白诱导的平滑肌细胞亲环素A表达的影响  

Effect of Amlodipine on Cyclophilin A Expression in Smooth Muscle Cells Induced by Oxidized Low Density Lipoprotein

作　　者：周志刚[1,2] 涂剑[3] 王北冰[4] 尹凯[2,4] 孟军[2] 涂玉林[2] 

机构地区：[1]南华大学附属第一医院,湖南省衡阳市421001 [2]南华大学心血管病研究所,湖南省衡阳市421001 [3]南华大学药理学教研室,湖南省衡阳市421001 [4]南华大学诊断学教研室,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2008年第11期865-868,共4页Chinese Journal of Arteriosclerosis

基　　金：湖南省医药卫生基金(B2007101);湖南省教育厅基金(07C635)

摘　　要：目的观察氨氯地平对氧化型低密度脂蛋白诱导的平滑肌细胞亲环素A表达的影响,以进一步探讨氨氯地平对动脉粥样硬化的作用机制。方法用不同浓度氨氯地平(0、0.1、1.0和10.0μmol/L)预孵育平滑肌细胞1h后,与氧化型低密度脂蛋白共同孵育,逆转录聚合酶链反应和Western blotting法检测细胞亲环素AmRNA及蛋白的表达。结果氧化型低密度脂蛋白抑制平滑肌细胞亲环素AmRNA及蛋白的表达,而氨氯地平预处理可上调亲环素A的表达。结论氨氯地平可逆转氧化型低密度脂蛋白抑制平滑肌细胞亲环素A表达的作用。Aim To investigate the effect of amlodipine on the expression of Cyclophilin A in smooth muscle cells induced by oxidized low density lipoprotein （ox-LDL） , so as to approach the mechanism of amlodipine in atherosclerosis. Methods Smooth muscle cells were treated with ox-LDL in different concentration or different time. Based on that, the cells were pretreated with amlodipine at different concentrations （0, 0.1, 1.0 and 10.0 μmol/L） and then incubated with ox-LDL, RT-PCR and Western blotting were used to detected the expression of Cyclophilin A in the cells.Results Tile expression of Cyclophilin A in smooth muscle cells induced by ox-LDL was obviously up-regulated by amlodipine in a dose-dependent manner. Conclusion Amlodipine may reverse the role of ox-LDL on the Cyclophilin A expression in smooth muscle cells.

关 键 词：病理学与病理生理学 亲环素A 氧化型低密度脂蛋白 平滑肌细胞 氨氯地平 

分 类 号：R363[医药卫生—病理学]