12株乳杆菌益生特性的研究  

Screening of probiotic properties of twelve strains of lactobacilli by in vitro techniques

作　　者：许荣誉[1] 庄建良[1] 张海云[1] 

机构地区：[1]福建医科大学附属泉州第一医院肿瘤外科,福建泉州362000

出　　处：《医学研究生学报》2008年第12期1246-1249,共4页Journal of Medical Postgraduates

基　　金：福建省2008年度自然科学基金资助项目(批准号:2008J0310)

摘　　要：目的:对12株乳杆菌的益生特性进行研究。方法:采用活菌计数法对12株乳杆菌的耐酸、耐胆盐特性进行筛选,采用琼脂打孔扩散法检测其抑菌活性,采用细胞培养革兰染色计数法检测其黏附性能。结果:12株乳杆菌对pH 2.5的强酸和0.3%高胆盐环境均有一定的耐受性,对引起肠道感染的几种病原菌都有不同程度的拮抗,其中约氏乳杆菌JCM1022抑菌谱较广,尤其是对大肠杆菌和肠炎沙门菌有很强的抑菌作用。而罗伊乳杆菌JCM1081对肠上皮样细胞Caco-2具有很强的黏附力。结论:约氏乳杆菌JCM1022和罗伊乳杆菌JCM1081可作为益生菌候选菌株应用于医药领域。Objective ： To study the probiotic properties of 12 selected strains of lactobacilli. Methods ： The probiotic properties of 12 selected strains of lactobacilli were investigated for their resistance to pH 2.5 and 0.3% oxgall, their adhesion to Caco-2 cells and antimicrobial activity against enteric pathogenic bacteria. Results： The 12 strains all showed relatively high resistance to acid and bile salt and varied antimicrobial activities against pathogenic bacteria of intestinal infection. Lactobacillusjohnsonii JCM1022 exhibited a broad inhibitory effect on enteric pathogenic bacteria, especially on enteropathgenic E. coliand Salmonella enteritidis, while Lactobacillus reuteri JCM1081 possessed a high activity of adhesion to Caco-2 cells （495.07 ±80.03）. Conclusion： Among the 12 strains, Lactobacillusjohnsonii JCM1022 and reuteri JCM1081 are qualified candidates for clinical application.

关 键 词：乳杆菌 耐受性 抑菌 黏附 

分 类 号：R378.2[医药卫生—病原生物学]