26例喉鳞状细胞癌微卫星不稳定性及杂合性丢失研究  

Microsatellite Instability and Loss of Heterozygosity in 26 Cases with Laryngeal Squamous Cell Carcinomas

作　　者：龚海珍[1] 赵佳[1] 祝威[2] 刘冰[1] 

机构地区：[1]吉林大学基础医学院医学遗传学教研室,吉林长春130021 [2]吉林大学第一医院,吉林长春130021

出　　处：《中国肿瘤》2009年第1期57-60,共4页China Cancer

基　　金：吉林省科技厅基金资助项目(200505187)

摘　　要：[目的]在喉癌易感染色体区域8p23.2、9p21.1、3p24.3筛查肿瘤相关基因,探讨CSMD1基因与喉鳞癌的相关性。[方法]在8p23.2﹑9p21.1﹑3p24.3区域选择D8S518﹑D8S264﹑D9S251、D3S2303四个微卫星多态性标记,应用聚合酶链反应(PCR)—变性聚丙烯酰胺凝胶电泳—银染法对26例喉鳞癌进行微卫星不稳定性及杂合性丢失分析。[结果]4个多态性标记中的3个有不同程度的微卫星不稳定性及杂合性丢失。其中D9S251位点MSI检出率为19%。D8S518位点LOH发生率为5%,MSI的发生率为10%,总的突变率为15%。D8S264位点MSI发生率为9.5%。[结论]8p23.2﹑9p21.1区域可能存在与喉鳞癌发生有关的基因,CSMD1基因可能是一个与喉鳞癌有关的抑癌基因。[Purpose] To screen the cancer related genes at 8p23.2, 9p21.1, 3p24,3 chromosomal region and to explore the correlation between CSMD1 gene and laryngeal squamous cell carcinoma. [Methods] Four mierosatellite polymorphism markers at 8p23.2, 9p21.1, 3p24.3 chromosomal region in 26 cases with laryngeal squamous cell carcinoma were selected to analyze loss of heterozygosity （LOH） and microsatellite instability （MSI） by polymerase chain reaetion-polyacrylamide urea gel electrophoresis（PCR- PAUGE）-DNA silver staining method. [Results] Loss of heterozygosity and microsatellite instability at different degree were found at 3 of the 4 microsatellite polymorphism markers. Among them, the incidence of MSI at D9S251 was 19%. The incidence of LOH at D8S518 was 5%; MSI, 10%; and overall incidence, 15%. The incidence of MSI at D8S264 was 9.5%. [Conclusion] Harbor genes related to laryngeal sqaamous cell carcinoma might locate at 8p23.2, 9p21.1 chromosomal. CSMD1 gene might be a tumor suppressor gene related to laryngeal squamous cell carcinoma.

关 键 词：喉肿瘤 鳞状细胞癌 微卫星不稳定性 杂合性丢失 DNA 

分 类 号：R739.65[医药卫生—肿瘤]