激光共聚焦显微镜定量检测釉质脱矿的研究  

Quantitative measurement of content of enamel demineralization by confocal laser scanning microscopy

作　　者：行勇军[1] 刘鲁川[2] 韩旭 

机构地区：[1]成都军区总医院市内门诊部口腔科,四川成都610017 [2]第三军医大学大坪医院野战外科研究所口腔科,重庆400042 [3]韩医师口腔诊所,辽宁锦州121000

出　　处：《西南国防医药》2009年第1期62-63,F0004,共3页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的:探讨激光共聚焦显微镜(CLSM)在定量检测釉质脱矿中的适用性,寻求高效、精确的定量检测龋损的方法。方法:以去离子水作为空白对照组,而以0.1 mol/L乳酸的脱矿液(pH 4.6)为实验组,分别对4组釉质磨片连续脱矿24h、48 h及96 h后,以激光共聚焦显微镜定量评价各组釉质块脱矿差异。结果:随着脱矿时间延长,釉质脱矿面积显著增大(P<0.01),而脱矿区总的荧光强度显著升高(P<0.01)。结论:激光共聚焦显微镜可对釉质脱矿量进行高效、精确的定量检测,且具有操作简便、无放射污染等优点。Objective： To explore the feasibility of measurement of the content of enamel demineralization by confocal laser scanning microscopy （CLSM） and establish a fast, accurate method of quantitative measurement of caries lesion. Methods：The sections of human enamel in treated groups were exposed to 0.1 mol/L lactic acid solution（ （ pH 4.6） for 24,48,96 h at 25℃ ,while the deionized water was used in control group under the same conditions. Enamel demineralization was evaluated by CLSM. Results ： In treated group, both of area of fluorescent lesion （A） and total fluorescence （TF） increased significantly with time of demineralization passing（P 〈 0.01 ）. Conclusion：CLSM can be used to measure the content of enamel demineralization with high efficiency and accuracy. It is convenient to perform and has no risk of X - ray radiation.

关 键 词：龋 釉质脱矿 共聚焦 

分 类 号：R781.1[医药卫生—口腔医学]