微波辅助三氯乙酸浸提啤酒废酵母细胞中海藻糖的技术研究  

Research on the Lixiviation of Trehalose from Waste Beer Barm Cells by Trichloracetic Acid Associated with Microwave

作　　者：王晓丽[1] 林松毅[1] 李海霞[1] 刘静波[1] 

机构地区：[1]吉林大学军需科技学院营养与功能食品研究室,吉林长春130062

出　　处：《酿酒科技》2009年第1期23-26,共4页Liquor-Making Science & Technology

基　　金：吉林省科技厅自然科学基金资助农业重点项目(20070201)。

摘　　要：以啤酒废酵母细胞为原料,以海藻糖得率为衡量指标,对比分析得出:啤酒废酵母细胞中海藻糖分解酶适宜灭活方法为微波灭酶900W、4min;通过L(934)正交实验设计,优化出三氯乙酸溶液浸提海藻糖的最佳技术参数为:提取温度为65℃,料液比1∶22,浸提时间120min,海藻糖得率达到11.07%。The yield of trehalose from waste beer barrn cells was used as the measuring index in the experiments. 4 rain 900 W microwave could exterminate the activity of decomposing enzyme of trehalose in waste beer barm cells. The optimum technical parameters were summed up as follows through L9 （34）orthogonal experiments： extraction temperature at 65 ℃, solid-liquid ratio as 1：22 and lixiviation time was 120 min. As a result, the yield oftrehalose could reach up to 11.07 %.

关 键 词：综合利用 分离提取 啤酒废酵母 微波 海藻糖 

分 类 号：TS261.9[轻工技术与工程—发酵工程]