体外保存兔甲状软骨活性的实验研究  

Viability of rabbit thyroid cartilage preserved in vitro

作　　者：杨宪法[1] 赵大庆[2] 崔鹏程[1] 徐凯[1] 

机构地区：[1]第四军医大学唐都医院耳鼻咽喉-头颈外科,陕西西安710038 [2]第四军医大学西京医院耳鼻咽喉-头颈外科,陕西西安710033

出　　处：《第四军医大学学报》2009年第1期73-75,共3页Journal of the Fourth Military Medical University

基　　金：陕西省科技攻关项目[2005K11-G4(3)]

摘　　要：目的:研究如何在体外长期保存兔甲状软骨块的活性.方法:取兔甲状软骨15块,软骨大小约5mm×2mm×1mm,将所取软骨块分别置于4℃,-80℃冷冻及RPMI-1640液,于37℃培养保存,于保存24h,7,14,21,30d时取样品,分别用HE,AB/PAS及Masson三色染色和台盼蓝排斥试验检测软骨活性.结果:用RPMI-1640培养液保存的兔甲状软骨,在整个保存期中活性保持较好,软骨细胞活性率保持在85%左右;-80℃冷冻保存7d时软骨基本失去活性,4℃保存软骨在保存30d时软骨活性明显降低.结论:与低温保存方法相比,用组织培养法能较好地保存兔甲状软骨块活性.AIM： To study the possibility of vital rabbit thyroid cartilage preservation in vitro using different methods. METHODS ：The normal rabbit thyroid cartilages （n = 15 ） were obtained and the size of cartilage fragments was about 5 mm × 2 mm × 1 mm. The fragments were divided into 3 groups at random and then were separately preserved at 4℃, - 80℃ and in RPMI- 1640 medium at 37℃. The viability of the cartilage grafts was compared after 1, 7, 14, 21 and 30 d, using histological method （ HE, AB/PAS, Masson stainings） and trypan blue dye exclusion test during the whole 30 d storage duration. RESULTS： The viability of catilage samples kept in RPMI-1640 medium was reserved best （about 85% ）. Cartilages kept at 4℃ for 30 d showed a quick decrease of viability, and the ones preserved at - 80℃ for 7 d lost their viability. CONCLUSION： Compared with low-temperature preservation, tissue culture method can successfully keep the viability of cartilages for a long time. It may offer a new vital cartilage preservation method for clinical application.

关 键 词：甲状软骨 组织培养 保存 

分 类 号：R318[医药卫生—生物医学工程]