发光细菌法检测水产品中氯霉素体系的建立  

Construction of a System to Detect Chloramphenicol Residues in Aquatic Foods by Luminescent Bacteria

作　　者：王亚群[1] 王静雪[1] 林洪[1] 朱兰兰[1] 王高燕[1] 

机构地区：[1]中国海洋大学食品安全性实验室,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2009年第1期66-70,共5页Periodical of Ocean University of China

基　　金：国家自然科学基金重点项目(30500384);国家高技术研究发展计划项目(2006AA09Z431)资助

摘　　要：从青岛近海分离的多株发光细菌中筛选出1株对氯霉素敏感的鳆发光杆菌Photobacterium leiognathiYL,接种单菌落快速培养,制备出适宜检测的菌悬液,添加不同浓度的氯霉素溶液,研究氯霉素浓度与细菌发光强度抑制率之间的关系。通过控制菌体起始发光强度、菌液与氯霉素作用时间,建立发光细菌检测水产品中氯霉素体系。结果表明,当菌体的起始发光强度控制在(2.0～4.0)×105、菌液与氯霉素的最佳作用时间为30 min时,氯霉素浓度与细菌发光强度抑制率呈良好的线性关系。该方法的线性范围为0.1～1.0 ng/mL,相关系数R2为0.989,该体系对氯霉素的检测灵敏度可以达到0.1 ng/mL,可以作为水产品中痕量氯霉素残留的1种快速、灵敏的检测方法。在水产品的氯霉素加标回收试验中,鱼肉中添加浓度为0.1～1.0 ng/g时,回收率在40.34%～114.26%之间。A marine bioluminescent bacterium, strain Photobacterium leiognathi YL was selected as the indicator for detecting Chloramphenicol（CAP） in seafood . The optimum interaction time was approximately 30 minutes, the initial luminescence of YL was （2.0- 4.0） × 10^5. A good linear relationship was observed between the CAP concentration and the inhibiting effects of the luminescent bacteria, the linear range was from 0.1 to 1.0 ng/mL with an R^2 of 0. 989. This system can detect trace CAP as low as 0.1 ng/mL, and it can be used as a rapid and convenient screen method for detecting CAP in seafood. The average recoveries were 40.34 to 114.26 % when samples were spiked with 0.1 to 1.0 ng/g of CAP.

关 键 词：发光细菌 水产品 氯霉素 快速检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]