血迹的光度法测定及其在物证分析中应用  

Application of Photometric Analysis of Blood Stain to Evidence Analysis

作　　者：胡秋娈[1] 刘继伟[1] 冯爱青[1] 陈伟[2] 

机构地区：[1]洛阳师范学院生命科学系,洛阳471022 [2]北京市公安局刑事科技研究所,北京100007

出　　处：《理化检验（化学分册）》2009年第1期70-72,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：河南省自然科学基金项目(No.0411021600);河南省教育厅自然科学基金项目(200510482009)

摘　　要：在pH 2.4的C-L缓冲介质中人血清蛋白与氯磺酚S反应生成蓝色复合络合物,其吸收峰位于635 nm波长处,摩尔吸光率(ε635)为2.49×105L·mol-1·cm-1,算得标准工作曲线的线性回归方程为A=3.66×10-3C-0.026,相关系数为1.000,人血清蛋白质量浓度在160 mg·L-1以内呈线性关系。方法的最低检测浓度为0.25 g·L-1,基于以上结果,提出应用上述方法于物证分析中测定血迹中人血清蛋白的含量。It was found that in a Clark-Lubs buffer medium of pH 2. 4, human serum albumin （HSA） in blood stain reacts with ehlorosulfophenol S to form a blue colored compound complex having its absorption maximum at 635 run and value of molar absorptivity of （ε635） 2. 49 × 10^5 L · mol^-1 ·cm^-1. Linear regression equation of the standard working curve obtained was as follows： A=3.66 × 10^-3 C-0. 026 （r= 1. 000）. Range of Iinearity within 160 mg · L^-1 of HSA was obtained. Minimum detectable concentration of the method was found to be 0. 25 g ·L^-1. Based on the facts mentioned above, the method was proposed to be used in the evidence analysis for determination of HSA in blood stain.

关 键 词：光度法 物证分析 人血清蛋白 血迹 

分 类 号：O657.32[理学—分析化学]