褐牙鲆精子生理特性及超低温冷冻保存  

Study on physiological characteristics and cryopreservation of spermatozoa in Paralichthys olivaceus

作　　者：章龙珍[1] 闫文罡[1,2] 庄平[1] 黄晓荣[1] 徐滨[1,2] 江琪[1,2] 

机构地区：[1]中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海200090 [2]上海高校水产养殖E-研究院,上海海洋大学,上海201306

出　　处：《上海海洋大学学报》2009年第1期21-27,共7页Journal of Shanghai Ocean University

基　　金：国家科技基础条件平台(2006DKA30470-004);农业部海洋与河口渔业重点开放实验室开放课题(开重-03-01);上海市科委基础重大专项(06dj14003)

摘　　要：对褐牙鲆精子部分生理特性进行了研究,结果表明,褐牙鲆精浆pH值为(7.90±0.26),精子密度为(1.03×1010)/mL,精浆渗透压为(308.50±7.85)m Osm/kg,K+在精浆中的浓度为(27±3)mmol/L,Na+在精浆中的浓度为(71±5)mmol/L。对不同盐度和pH与褐牙鲆精子活力和寿命的关系进行了研究,结果表明,在盐度为35,pH为8.0时,精子的活力最高,分别达到(93.33±5.67)%和(89±3.33)%,与其生活环境相适应;褐牙鲆精子寿命为(23±2)min,与已报道其它海水鱼精子寿命相近。分别采用室温(25℃)和低温(4℃)保存精子,发现精子在室温条件下,可存活4 d,在低温条件下可以存活7 d,证明低温可有效延长精子存活时间。另外,分别采用4种不同稀释液(S1、S2、S3、S4)与4种不同抗冻剂(DMSO、EG、Gly、PG)配制成冷冻保存液对褐牙鲆精子进行超低温冷冻保存研究。结果表明,以S3液作为稀释液,16%的DMSO作为抗冻剂,利用快速降温法对精子进行超低温冷冻保存,38℃水浴快速解冻,解冻后的精子获得最高的活力为(84±3.67)%,此方法可用于褐牙鲆精子的长期保存。Some physiological characteristics of Paralichthys olivaceus sperm were studied. The results showed that the pH of Paralichthys olivaceus sperm was （7.90 ±0.26）, density of sperm was （ 1.03 × 10^10）/ml, the osmolality of semen was （308.50 ± 7.85 ） m Osm/kg. The concentration of K^＋ in semen was （27 ± 3 ） mmol/ L, concentration of Na ~ was （ 71± 5 ） mmol/L. The relation between salinity （ or pH ） and Paralichthys olivaceus sperm motility was also studied, the results demonstrated that sperm had the best motility of （93.33 ± 5.67） % under salinity of 35 and motility of （ 89 ± 3.33 ） % at pH 8.0, which adapt to the environment. The life-span of Paralichthys olivaceus sperm was （23±2） min, and it was similar to the life-span of other reported marine fishes. The life-span of Paralichthys olivaceus sperm was 4 d under room temperature （25℃ ）, whereas it was 7 d under low temperature （4 ℃ ）. It shows that low temperature could effectively extend the survival time of sperm. Four different diluters （ S1, S2, S3, S4） and cryoprotectants （ DMSO, EG, Gly, PG） were used for cryopreservation of Paralichthys olivaceus sperm study, results showed thawed sperm （38 ℃ water） had highest motility （ 84 ± 3.67 ） % under the S3 diluter with 16% DMSO as cryoprotectant. This method can be used for long-term preservation of Paralichthys olivaceus sperm.

关 键 词：褐牙鲆 精子 活力 稀释液 抗冻剂 

分 类 号：S917[农业科学—水产科学]