鹰嘴豆籽粒贮藏蛋白双向电泳的研究  

Two-DE analysis of chickpea seed storage of proteins

作　　者：王显生[1] 高文瑞[1,2] 石庆华[3] 张桦[3] 张巨松[3] 李建贵[4] 何小玲[1] 麻浩[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095 [2]江苏省农业科学院蔬菜所,江苏南京210014 [3]新疆农业大学农业生物技术重点实验室,新疆乌鲁木齐830052 [4]新疆农业大学林学院,新疆乌鲁木齐830052

出　　处：《湖南农业大学学报（自然科学版）》2008年第6期635-639,672,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家科技支撑计划项目(2006BAD09A04;2006BAD09A08;2007BAC15B06);国家自然科学基金项目(30860152);高等学校学科创新引智计划项目(教育部);江苏省博士后科研资助(0801048B);南京农业大学作物遗传与种质创新国家重点实验室开放基金课题(ZW2005005)

摘　　要：以鹰嘴豆为材料,通过对样品制备、电泳条件的优化,建立其籽粒贮藏蛋白的双向电泳技术,并通过电泳图谱的分析,了解贮藏蛋白等电点和相对分子质量的分布情况.结果表明,经考马斯亮蓝染色获得贮藏蛋白斑点约380个,等电点范围在pH4.0～8.0,其中大部分集中在pH6.0～7.0;鹰嘴豆籽粒贮藏蛋白质的相对分子质量为11000～120000,表现出2个分布峰,一个在30000～40000,另一个在50000～60000;鹰嘴豆与大豆籽粒贮藏蛋白的分子质量和等电点分布基本相似.Two-dimensional electrophoretic technology for chickpea seed storage of proteins was established through optimization experiment of sample preparation and some key electrophoresis conditions, and used to investigate their isoelectric points and molecular mass range. Results showed that 380 proteins spots were detected on the 2-DE maps by coomassie brilliant blue-stained, and their isoelectric points distributed between pH4.0-8.0, with most between pH 6.0-7.0. Their relative molecular masses were between 11 000-120 000 with two peaks. One peak is in the range of 30 000-40 000 and another is in the range of 50 000-60 000. Both chickpea and soybean seed storage proteins have similar distribution of molecular mass and isoelectric points.

关 键 词：鹰嘴豆 贮藏蛋白 双向电泳 

分 类 号：Q949.751.9[生物学—植物学] Q503