抗K88-K99-^(987)P多联蛋黄抗体活性的测定及其特性研究  

The determination of anti-K88-K99-^(987)P of egg yolk antibody and its characteristic

作　　者：徐榕榕[1] 贾莉[1] 陆建安[1] 杨严俊[2] 薛文君 邹三元 

机构地区：[1]食品科学与技术国家重点实验室 [2]江南大学食品学院,江苏省无锡市214122 [3]宜兴天石饲料有限公司

出　　处：《中国饲料》2009年第1期26-29,32,共5页China Feed

基　　金：国家十一五科技支撑计划"液态蛋与专用蛋粉开发与产业化示范项目"(2006BAD05A17);江苏省科技攻关项目"复合多价高效蛋黄抗体水性免疫球蛋白项目"(BE2005341)

摘　　要：本文对经弗氏佐剂、白油佐剂混合免疫产蛋鸡得到的抗K88-K99-987P蛋黄抗体(IgY)的活性测定方法进行了研究,并对IgY的生物活性及其特性进行了初步的研究。试验结果为:(1)采用ELISA法测定表明:采用白油佐剂和弗氏佐剂苗免疫产蛋鸡所制得的抗K88-K99-987PIgY比对照组的IgY分别提高32和16倍。(2)IgY的耐热试验表明:纯化的IgY和蛋黄粉中的IgY其活性在20～50℃时差别不大,但当温度继续升高时,蛋黄粉中的IgY的耐热性比纯化的IgY好。(3)IgY的耐酸、碱试验表明:37℃时,当pH为2～4和8～10时,蛋黄粉中IgY活性的影响比纯化的IgY的活性影响显著。(4)胃蛋白酶水解IgY的试验表明:当pH在2.0和3.0时,胃蛋白酶对IgY的水解作用显著;当pH为4.0～6.0时,胃蛋白酶对IgY的水解作用明显降低。在pH为2.0和3.0时,蛋黄粉中IgY的抗胃蛋白酶水解的能力要高于纯化的IgY。(5)K88抑菌试验表明:当IgY的添加量在1～10mg/mL时,能有效抑制细菌的生长繁殖。The determination of activity of immunoglobulin yolk （IgY）from the laying chickens immunized by the combination of K88-K99-^987P with different commercial adjuvant, Freund＇s adjuvant and white oil adjuvant were investigated.The bioactivity and characteristics of IgY were also studied in this paper.The results showed： （1）The ELISA test showed the IgY against K88-K99-^987P titers for Freund＇s adjuvant vaccines were 32 times higher,whereas for the white oil adjuvant vaccines were 16 times higher than the titer of control. （2）There was no significant difference in the activity between purified IgY and unpurified IgY in powder-form when the temperature from 20 - 50℃ were tested.However,when the temperature increased further,the resistance of unpurified IgY in powder-form against heat was better.（3）The activity of IgY had a distinct effect at 37 ℃when the pH ranged from 2 to 4 and from 8 to lO,respectively.The hydrolyzation of IgY by pepsin was significant at pH 2.0 and 3.0,whereas the hydrolyzation became weak when pH ranged from 4.0 to 6.0. The resistance to pepsin hydrolyzation of unpurified IgY in powder-form was better than purified IgY.The inhibition effect of IgY on K88 showed the growth of K88 was inhibited when IgY were added into the culture medium at 1 - 10 mg/mL.

关 键 词：蛋黄抗体 ELISA法 

分 类 号：S816.17[农业科学—饲料科学]