胚胎期X线照射致大鼠皮质-纹状体区异常的研究  

Studies on abnormalities of the embryonic rat cortex-striatum following prenatal X-ray irradiation

作　　者：李洪鹏[1] 白旭东[1] 川野仁 佟晓杰[1] 

机构地区：[1]中国医科大学基础医学院人体解剖学教研室,沈阳110001 [2]东京都神经科学综合研究所发生形态研究部门,日本183-8526

出　　处：《神经解剖学杂志》2009年第1期41-46,共6页Chinese Journal of Neuroanatomy

基　　金：辽宁省教育厅基金(No.2008778)资助项目

摘　　要：为探讨胚胎期X线照射致大鼠皮质-纹状体区的异常,本研究选取妊娠14d(E14)大鼠接受X线(剂量为1.0Gy)照射。照射前2h经尾静脉注入5-溴-2-脱氧尿嘧啶(BrdU),用以标识E14的神经细胞的发生。取不同时期(E14-E18)胚脑用于HE染色、BrdU免疫组化染色及Fluoro Jade B(FJB)染色观察。结果显示:HE染色观察到E14大鼠受X线照射后背侧端脑有大量细胞脱落入侧脑室并在2d后被完全清除,而腹侧的皮质-纹状体区在照射后2d形成空洞并于4d后修复。BrdU标识显示腹侧端脑的外侧节隆起的神经细胞移动类似于正常对照组,2d后阳性细胞分布在皮质-纹状体区形成的空洞周围,4d后移动到腹侧皮质板。Fluoro Jade B染色显示照射后1d在皮质-纹状体区有大量阳性细胞,2d后显著减少。本实验结果提示:E14大鼠受X线照射引起外侧节隆起的神经细胞在移动过程中死亡并导致皮质-纹状体区形成空洞,从而引起内囊发生异常。To approach the abnormalities in embryonic rat cortex-striatum following prenatal X-ray irradiation, the pregnant rats were ex- posed to X-ray irradiation （ 1.0 Gy） at embryonic day 14 （E14）. In addition, the pregnant rats were injected with 5-bromo-2-deoxyuridine （BrdU） via tail vein 2 h before X-ray irradiation to label neural cells that were generated at E14. The abnormal development of cerebral cortex was examined by hematoxylin-eosin （HE） staining, BrdU-labeling and Fluolo Jade B （FJB） staining. The results obtained were as follows： by HE staining, many cells in dorsal telencephalon shed into the lateral ventricle and disappeared after 2 days following X-ray irradiation at E14. In the ventral eortex-striatum, the cystic cavities were formed after 2 days and repaired 4 days following X-ray irradiation. BrdU labeling indicated that the positive cells in the lateral ganglionic eminence migrated in a similar way to those in control group 6 h following X-ray irradiation, the positive cells were observed to be around the cystic cavities in cortex-striatum 2 days later and migrated to the ventral cortical plate 4 days later. By FJB staining, many positive cells were seen to be in the cortex-striatnm following X-ray irradiation 1 day later, and decreased after 2 days. The present investigation suggests that cell death of the lateral ganglionic eminence oecurrs during cellular migration and results in the cystic cavities in the cortex-striatum, and leads to abnormal development of the internal capsule following prenatal X-ray irradiation at E14.

关 键 词：X线照射 外侧节隆起 皮质-纹状体区 5-溴-2-脱氧尿嘧啶 FLUORO JADE B 大鼠 

分 类 号：R363[医药卫生—病理学]