高效纤维素降解菌的筛选及其系统发育分析  

SCREENING OF CELLULOSE-DEGRADING FUNGI AND THEIR PHYLOGENETIC ANALYSIS

作　　者：张宇[1,2] 尹依婷[3] 许敬亮[1] 袁振宏[1] 庄新姝[1] 

机构地区：[1]中国科学院广州能源研究所中国科学院可再生能源与天然气水合物重点实验室,广州510640 [2]中国科学院研究生院,北京100039 [3]辽宁大学生命科学系,沈阳110036

出　　处：《太阳能学报》2009年第1期103-106,共4页Acta Energiae Solaris Sinica

基　　金：国家高技术研究发展计划(863)计划(No.2007AA05Z406;2007AA1007024);中国科学院知识创新工程重大项目(No.KSCX1-YW-11-A3);中国科学院广州能源研究所所长创新基金项目-人才引进专项(No.0607681001)

摘　　要：从刚果红平板上筛选到S41、Z3b、Z28和Z41共4株纤维素酶高产菌株,通过形态观察,生理生化特性分析以及系统进化分析等,鉴定出S41和Z41属于木霉属(Trichoderma sp.),Z3b属于曲霉属(Aspergillus sp.),Z28属于肉座菌属(Hypocrea sp.);4株菌的CMC酶活,FPA酶活和β-葡萄糖苷酶活几乎均高于绿色木霉(Trichderma viride) AS3.2774,葡萄糖对4株菌的β-葡萄糖苷酶均有一定的反馈抑制作用,对Z28的β-葡萄糖苷酶抑制作用较低。Four fungi named S41, Z3b, Z28 and Z41 were isolated froin soil, capable of producing cellulase with high activity. According to their phenotypic features, physiological and biochemical characteristics, and phylogenetic analysis based on the sequence of 18S rDNA, ZA1 and S41 was identified as Trichoderrna sp., Z3b was identified as Aspergillus sp., Z28 was identified as Hypocrea sp. The activity of CMC, FPA, and β-glucosidase of the isolates is almost higher than that of Trichderma viride AS3. 2774. Still, studies with tolerance of β-glucosidase to glucose found that β-glucosidase produced by Z28 is the most highly glucose-tolerant of the four fungi.

关 键 词：纤维素酶 Β-葡萄糖苷酶 系统发育分析 

分 类 号：TK511.4[动力工程及工程热物理—热能工程]