全自动发酵罐小试生产胆固醇氧化酶发酵条件的研究  

Fermentation production of cholesterol oxidase by Rhodococcus sp.R_(14-2) in fermentor

作　　者：王成涛[1] 曹雁平[1] 王静[1] 张慧[1] 孙宝国[1] 

机构地区：[1]北京工商大学化学与环境工程学院,北京100037

出　　处：《食品工业科技》2009年第1期159-161,164,共4页Science and Technology of Food Industry

基　　金：北京市优秀人才培养专项(20061D0500300137);黑龙江省教育厅科技项目(10531125)

摘　　要：目的:为了将胆固醇氧化酶应用于低胆固醇系列食品开发和胆固醇测定中,研究优化了菌株Rhodococcus SP.R14-2发酵生产胆固醇氧化酶(COX)的工艺条件。方法:通过测定比较COX酶活力,利用全自动发酵罐小试生产COX,研究发酵温度、pH、乳化剂、通风量和搅拌速率等因素对COX产量的影响。结果:Rhodococcus SP.R14-2的生物生长量和产酶是部分偶联的,胞外COX酶的合成高峰比生物量高峰滞后约12h。发酵过程中分段控制搅拌转速和通气量分别为:发酵前10h,200r/min和4L/min;10～50h,300r/min和5L/min;50h后,250r/min和4.5L/min,COX达到1.58U/mL的最高量。结论:发酵温度、培养基pH、通气量及搅拌速度等因素影响其生物量和COX酶合成,采用分段式控制,有利于COX产量的提高。Objective：the interest in cholesterol oxidase （COX）is due to it＇ s industrial importance and it＇ s wide utilization in the determination of cholesterol in food and blood serum.Methods.Rhodococcus SP.R14-2 was grown in a batch fermentor.The effect of cultivation temperature, pH of the culture medium,Tween-80, aeration rate and agitation speed on the production of COX by the microorganism were studied. Results.all of them effected it＇ s biomass and production of COX,and there were partly relationship in biomass of R14-2 and production of COX. Adjusting the operation variables could increase the production of COD effectively during the fermentation. By adjusting operation condition,a maximum COX activity of 1.58U/mL could be achieved at 66h.

关 键 词：胆固醇氧化酶 发酵罐 条件优化 分段调控 

分 类 号：TS201.25[轻工技术与工程—食品科学]