反义寡核苷酸对HL60细胞c-myc基因表达的抑制和诱导分化作用  

STUDY OF INHIBITION OF HL60 CELLS c-myc GENE EXPRESSION AND INDUCTION OF DIFFERENTIATION BY ANTISENSE OLIGODEOXYNUCLEOTIDE

作　　者：王松梅[1] 单易非[1] 高红阳 汤华[1] 

机构地区：[1]上海医科大学基础医学院生物物理学教研室

出　　处：《上海医科大学学报》1998年第3期167-170,共4页Journal of Fudan University(Medical Science)

摘　　要：目的研究ｃｍｙｃ反义寡核苷酸对ＨＬ６０细胞中ｃｍｙｃ基因的表达及ＨＬ６０细胞分化的影响。方法采用针对原癌基因ｃｍｙｃ的ｍＲＮＡ５’非翻译区的一个１８ｂｐ的反义寡核苷酸５’ｄ（ＣＴＴＣＴＣＧＡＧＧＣＡＧＧＡＧＧＧ）３’与人早幼粒白血病细胞系ＨＬ６０细胞共培养５ｄ，检测ｃｍｙｃｍＲＮＡ量的变化及其对ＨＬ６０细胞分化的影响。结果用ＮｏｒｔｈｅｒｎＢｌｏｔ检测出ＨＬ６０细胞ｃｍｙｃｍＲＮＡ的含量减少；细胞形态学变化以及非特异性酯酶（ＮＳＥ）染色、硝基四氮唑蓝（ＮＢＴ）还原试验均提示ＨＬ６０细胞出现了分化，部分细胞向粒细胞方向分化，部分细胞向单核细胞方向分化。结论ｃｍｙｃ反义寡核苷酸能够抑制ｃｍｙｃ基因的表达并能诱导ＨＬ６０细胞分化。PURPOSE To study the effect of an antisense oligodeoxynucleotide on the c myc gene expression and HL60 cells differentiation. METHODS Photomicrograph was used to show the morphology of the Wright's stained HL60 cells, Northern Blot to Measure the level of RNA, and NSE and NBT to detect the differentiation. RESULTS The expression of c myc gene mRNA was inhibited by the antisense oligodeoxynucleotide. Cell morpological changes and differentiation suggest that HL60 cells differentiate along two pathways: some into monocytes and some into granulocytes. CONCLUSIONS C myc antisense oligonucleotide can inhibit the expression of c myc mRNA and induce the differentiation of HL60 cells.

关 键 词：C-MYC基因 HL60细胞 反义寡核苷酸 分化 

分 类 号：R733.702[医药卫生—肿瘤] R979.12[医药卫生—临床医学]