混合添加乙醇和H_2O_2对嗜热子囊菌产过氧化氢酶的影响  

Effects of adding mixture of ethanol and hydrogen peroxide on production of catalase by Thermoascus aurantiacus

作　　者：芦国军[1,2] 华兆哲[1,2] 堵国成[1,2] 陈坚[1,2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江南大学生物工程学院,江苏无锡214122

出　　处：《工业微生物》2009年第1期42-47,共6页Industrial Microbiology

基　　金：国家重点基础研究发展规划项目(973计划)(No.2007CB714306);国家自然科学基金项目(20676056)

摘　　要：在以嗜热子囊菌(T.aurantiacus WSH03-01BC)生产过氧化氢酶的7L罐发酵研究中,发现混合添加适量的乙醇(75%)和H_2O_2可以促进菌体产酶。在发酵36h和48h分别添加0.8%(v/v)的乙醇时,酶活比对照提高了34.3%;当添加乙醇的总量超过2.4%时,对菌体的生长及产酶有明显的抑制作用;在发酵36～60h恒速流加1.6%的乙醇,CAT的酶活达到2519U/mL,单位细胞产酶能力提高了47.3%;在发酵36h～60h恒速流加1.6%的乙醇并在48h混合添加0.4%的H_2O_2时,CAT的酶活达到2786U/mL,比对照提高了50.1%。The effects of adding the mixture of ethanol（75 % ） and H2O2 on the production of catalase （CAT） by T. aurantiacus WSH03-01 were investigated in a 7L fermentor. The addition with appropriate mixture of ethanol and H2O2 enhanced the production of CAT. Compared with that of the control, CAT activity increased 34.3 % by adding 0.8 % （v/ v） ethanol to the broth at 36h and 48h. When the total ethanol in the fermentation liquor exceed 2.4% （v/v）, the production of CAT and the growth of T. aurantiacus WSH03-01 were inhibited significantly. When 1.6% ethanol was added to the broth by fed-batch with constant rate from 36h to 60h , the CAT activity reached 2519U/mL, and the specific productivity of CAT increased 47.3 % compared with that of the control. When 1.6 % ethanol was added with constant rate from 36h to 60h and 0.4% H2O2 （v/v） was added at 48h, the CAT activity reached 2786U/mL which was 50.1% higher than that of the control （1856U/mL）.

关 键 词：过氧化氢酶 嗜热子囊菌 乙醇 过氧化氢 混合添加 

分 类 号：TQ925[轻工技术与工程—发酵工程]